We narrowed to 165,634 results for: DES

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pDEST-SorLA-v2

Plasmid

#154900

Purpose

Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2Q2

Plasmid

#15801

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-Tpt1

Plasmid

#42571

Depositor

Stan Fields, Jay Hesselberth

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

Tpt1

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pGGDestTol2LC-1sgRNA

Plasmid

#64239

Purpose

destination vector for one U6x:sgRNA cassette

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_pK7WG2_pRPS5A_CTP OleGFP

Plasmid

#171723

Purpose

ptpTALECD vector assembly, Destination vector;

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2

Expression

Plant

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pFRT-DestFLAGHA_HuD

Plasmid

#65760

Purpose

constitutively expresses FLAG-HA-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2R2

Plasmid

#15806

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Zebrabow-GateDest

Plasmid

#118221

Purpose

For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L - ccdB survival cassette

Use

Zebrafish expression

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_RNAseH1

Plasmid

#65785

Purpose

expresses RFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

RFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_HuC

Plasmid

#65756

Purpose

expresses FLAGHA-tagged HuC in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL3 (ELAVL3 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR

Plasmid

#31222

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdTom (CD4 Aequorea victoria, Human, Fly)

Tags

CD4 and tdTomato

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST26-N-FLAG

Plasmid

#79549

Purpose

Destination vector containing an N-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits