We narrowed to 15,050 results for: pET

1. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-PSAT

   Plasmid

   #74115

   Purpose

   Expression of PSAT

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   pET28a-PSAT (PSAT1 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET151-Vps4

   Plasmid

   #87733

   Purpose

   Bacterial expression of full-length S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-3c-rhVTN-C

   Plasmid

   #30224

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with C terminus cleavage (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478

   Available Since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MinC

   Plasmid

   #133628

   Purpose

   Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

   Insert

   His-MinC (minC E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-MRPP1

   Plasmid

   #67862

   Purpose

   bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   MRPP1 (TRMT10C Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   mitoch. form (contains aa 40-403)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(ICOS)

   Plasmid

   #177903

   Purpose

   Bacterial expression of his10-tagged human ICOS Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   ICOS (aa 162-199 only) (ICOS Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet-T7-Ct-WspR

   Plasmid

   #79166

   Purpose

   Dual expression of Ct biosensor and WspR WT under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   WspR (wspR P. areuginosa)
   T7-Spinach2-Ct
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEThT-SAHS2

   Plasmid

   #90026

   Purpose

   To express His-tagged SAHS2 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   SAHS2
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET16b-Mms2

    Plasmid

    #18893

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
    

    Tags

    HIS10

    Expression

    Bacterial

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pET22b-EC12WTC9AAvi

    Plasmid

    #47925

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis, Avi Tag, and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. YPet-Actin-7

    Plasmid

    #56628

    Purpose

    Localization: Actin, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    Actin (ACTB Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pETTEV-SENP8

    Plasmid

    #196361

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    SENP8/NEDP1
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-T5-mad32TAG

    Plasmid

    #188997

    Purpose

    Express madanin-1 with TAG at 32 position

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    madanin-1 with TAG at 32 position
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-T5-mad

    Plasmid

    #188998

    Purpose

    Express wildtype madanin-1

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    wildtype madanin-1
    

    Expression

    Bacterial

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. yPet_cp229-Binder

    Plasmid

    #179137

    Purpose

    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    yPet_cp229

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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