We narrowed to 14,883 results for: pET

1. yPet_cp229-Binder

   Plasmid

   #179137

   Purpose

   YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   yPet_cp229

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-T7-Ct-YhjH

   Plasmid

   #79167

   Purpose

   Dual expression of Ct biosensor and YhjH under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   yhjH
   T7-Spinach2-Ct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM33_CLTC_Clathrin propeller

   Plasmid

   #178471

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CLTC_Clathrin propeller (CLTC Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-OmpT

   Plasmid

   #68862

   Purpose

   construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

   Depositor

   Neil Kelleher

   Article

   Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

   Insert

   OmpT (ompT )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EcYbbN pET15b

   Plasmid

   #60690

   Purpose

   Recombinant protein expression of YbbN

   Depositor

   Mark Wilson

   Article

   Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

   Insert

   YbbN
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET16b-Mms2

   Plasmid

   #18893

   Depositor

   Cecile Pickart

   Article

   Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

   Insert

   Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
   

   Use

   Tags

   HIS10

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pET28-HADH2

   Plasmid

   #67863

   Purpose

   bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   HADH2 (HSD17B10 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT5H158Y-pET15b

   Plasmid

   #81032

   Purpose

   Bacterial expression of Human catalytic mutant SIRT5 protein

   Depositor

   David Lombard

   Article

   Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

   Insert

   Sirt5 (SIRT5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H158Y catalytic mutant

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a(cd226)

   Plasmid

   #177900

   Purpose

   Bacterial expression of his10-tagged human cd226 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD226 (aa 288-336 only) (CD226 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Beclin1 CC

    Plasmid

    #24403

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains the CC domain (aa 151-242)

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pET-CV-Ykt6

    Plasmid

    #168030

    Purpose

    Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-Ykt6(Q131-K190)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET30-2-PSPH

    Plasmid

    #83909

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    phosphoserine phosphatase (PSPH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b-Ava3855

    Plasmid

    #45743

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3855 (Ava_3855 Anabaena variabilis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET29b-EPN-01*

    Plasmid

    #80423

    Purpose

    For expression of EPN-01* in E. coli

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pETM-11-HsPABPC1_J

    Plasmid

    #146642

    Purpose

    Bacterial Expression of HsPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    HsPABPC1 (PABPC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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