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Plasmid#177839PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.DepositorTypeEmpty backboneUseBacterial gene replacementAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only
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MSCV-Myc-IRES-RFP
Plasmid#35395DepositorAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
Plasmid#47868PurposeThis plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.DepositorInsertsNmCas9
U6pr-tracrRNA
UseCRISPRTagsHA and NLSExpressionMammalianPromoterEF1a and U6Available SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-IRES-GFP
Plasmid#35396DepositorAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJW1347
Plasmid#154310PurposeDonor-cassette for SapTrapDepositorInsert30aa Linker for CT slot
UseCRISPRExpressionWormAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR mCHERRY v1 backbone
Plasmid#179778PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)
Plasmid#47870PurposeAll-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.DepositorInsertsNmCas9
U6pr-tracrRNA
OCT4 crRNA
UseCRISPRTagsHA and NLSExpressionMammalianAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR BFP v1 backbone
Plasmid#179779PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1759
Plasmid#154315PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker::TEV::AID*::3xFLAG for NT slot
UseCRISPRExpressionWormAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1348
Plasmid#154311PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker for NT slot
UseCRISPRExpressionWormAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_1
Plasmid#204672PurposeDual gRNA plasmid for UCK2 deletionDepositorAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_CDC20:161-P477
Plasmid#210888PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC20:S161-P477 (CDC20 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
ExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
ExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_EML4:250-877
Plasmid#210914PurposeInsect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.DepositorInsertEML4:I250-A877 (EML4 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationK283E natural variant (VAR_031726), I382V Natural…PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_ATG16L1:303-607
Plasmid#210942PurposeInsect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertATG16L1:G303-Y607 (ATG16L1 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 4, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_LRRK2:2141-2527
Plasmid#210901PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBacMam2-DiEx-LIC_LRRK2:2141-2527
Plasmid#210900PurposeInsect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsAENLYFQSHHHHHHHHHHDYKDDDDK and MExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterP10 and CMVAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_CDC40:236-578
Plasmid#210885PurposeInsect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC40:E236-W578 (CDC40 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1172
Plasmid#29301PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorAvailable SinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only