We narrowed to 9,135 results for: mel

Showing: 8781 - 8800 of 9135 results

Results per page:

Sort results by:

pAcUW51-CgE

Plasmid

#13762

Depositor

Pamela Bjorkman

Article

Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

Insert

HSV-1 glycoprotein E

Tags

His

Expression

Insect

Mutation

C-terminal region of the HSV-1 gE (KOS strain ect…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pJK520

Plasmid

#155386

Purpose

PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
PphlF-TA1 in dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-MAP2K1-K57N

Plasmid

#116427

Purpose

Lentiviral expression of MAP2K1 K57N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

K57N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-MAP2K1-P124S

Plasmid

#116428

Purpose

Lentiviral expression of MAP2K1 P124S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

P124S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-MAP2K1-S222A

Plasmid

#116429

Purpose

Lentiviral expression of MAP2K1 S222A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S222A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster vasa

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP CMV VHHG4

Plasmid

#182236

Purpose

Expresses GFP nanobody for stable expression in mammalian cells

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

anti-GFP nanobody

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT_TO_eGFP_ELAVL1

Plasmid

#106105

Purpose

Inducible gene expression of EGFP ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

NP_001410.2 (ELAVL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-mTrim21ΔPRYSPRY

Plasmid

#105518

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS2 (pBS0249)

Plasmid

#185139

Purpose

For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEMHE-mCherry-mTrim21ΔPRYSPRY

Plasmid

#105524

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-CDC50A-c3xFLAG

Plasmid

#215702

Purpose

Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CDC50A (TMEM30A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster nanos

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge