162,326 results
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Plasmid#221366PurposeMammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.DepositorInsertAnti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-Sth1Cas9
Plasmid#129552PurposeContains codon-optimized Sth1Cas9 and gRNA for streptomycesDepositorInsertSth1Cas9
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneUseTagsGSTExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
3` CC: Hygro – GFP-N – loxP – mScarlet
Plasmid#219564Purpose3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosomeDepositorInsert3` CC: Hygro – GFP-N – loxP – mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF1-aAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRA1B-Tango
Plasmid#66214PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRA1B (ADRA1B Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMIBlu
Plasmid#176621PurposeRetroviral transfer vector expressing TagBFPDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant
Plasmid#177130PurposeGenerate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1DepositorInsertYTHDC1 (YTHDC1 Human)
UseLentiviralTagsmClover3ExpressionMutationW428A/W377A (m6A binding defects), and siRNA resi…PromoterEf1aAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Negative Control Binder
Plasmid#179140PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptideDepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9. Addi…PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.D594H
Plasmid#82816PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationD594HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas ServiceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-msGFP2
Plasmid#135301PurposeEncodes a photostable monomeric superfolder GFPDepositorInsertmsGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
iLID-mCl3-MAP7N
Plasmid#190167PurposeExpresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertiLID-mClover3-MAP7N
UseTagsiLID-mClover3ExpressionMammalianMutationaa 1-309 from human MAP7 delta175-213PromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB-C5
Plasmid#28213Purpose5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unitDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2011AvailabilityAcademic Institutions and Nonprofits only