165,567 results

1. pEGFP C3-YAP2

   Plasmid

   #19055

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Marathon-RT pentamutant for Split-PE (pEH107)

   Plasmid

   #190110

   Purpose

   Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, N26R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBa.TfR.GFP

   Plasmid

   #45060

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Burack et al Neuron. 2000 May;26(2):465-72.

   Insert

   TfR (TFRC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta Actin

   Available Since

   Oct. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SBE-Luc

   Plasmid

   #16527

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   Smad binding element
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

5. mEmerald-Fascin-N-10

   Plasmid

   #54095

   Purpose

   Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. CYFIP1.mCherry

   Plasmid

   #122051

   Purpose

   Expresses human CYFIP1 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoBE4-SpRY

   Plasmid

   #226854

   Purpose

   Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   evoBE4max-SpRY
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_V5-TWIST1

    Plasmid

    #215030

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CmYLCV

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSW GFP IRES GFP

    Plasmid

    #133815

    Purpose

    Encodes EGFP that can be used as a control for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-GCaMP6m-Xn

    Plasmid

    #118976

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XN
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. VPS13B^Halo

    Plasmid

    #224584

    Purpose

    codon optimized Halo tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^Halo (VPS13B Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP-TMEM192-mKeima

    Plasmid

    #215358

    Purpose

    Probe to evaluate lysophagic activity

    Depositor

    Tamotsu Yoshimori

    Article

    Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

    Insert

    transmembrane protein 192 (TMEM192 Human)
    

    Use

    Retroviral

    Tags

    mKeima

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LARS_pNIC-Bio3

    Plasmid

    #153052

    Purpose

    Expression construct for producing Avi tagged Leucyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    LARS (LARS1 Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Not full length protein

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ExRai-AktAR2

    Plasmid

    #184047

    Purpose

    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    ExRai-AktAR2
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Expression

    Mammalian

    Promoter

    Mammalian Pol I

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB2-mScarlet-I

    Plasmid

    #104007

    Purpose

    Plasmid encoding mScarlet-I

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet-I
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIX403_UBAP2L_mCherry

    Plasmid

    #105286

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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