165,567 results

1. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-ERalpha

   Plasmid

   #215093

   Purpose

   Expresses HA-ERalpha in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ADAR2DD_E488Q

   Plasmid

   #205598

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   ADAR2 deaminase domain (ADARB1 Human)
   

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutation

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST2T18ms

   Plasmid

   #72647

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Available Since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HA-HIF2α wt-pBabe-Puro

   Plasmid

   #26055

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

   Plasmid

   #128455

   Purpose

   lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
   

   Use

   Lentiviral

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExchange_tdk_BT1311p-tetR-1

   Plasmid

   #90325

   Purpose

   Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   TetR
   

   Expression

   Bacterial

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWM_12x601_15bpLinker

   Plasmid

   #157786

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_15bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJS001

   Plasmid

   #188324

   Purpose

   PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::GFP::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmEGFP_a_tubulin_C1

    Plasmid

    #21039

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-TRIM24

    Plasmid

    #28138

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-TRE-C-prM-E-NS1

    Plasmid

    #175277

    Purpose

    AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Promoter

    tight TRE

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lox2272 Neo* lox2272

    Plasmid

    #168769

    Purpose

    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SLC3A1

    Plasmid

    #132201

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A1 (SLC3A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRM102

    Plasmid

    #44525

    Depositor

    Michael Grunstein

    Article

    Mann et al EMBO J. 1992 Sep;11(9):3297-306.

    Insert

    P(GAL10)-HHT2 P(GAL1)-HHF2
    

    Expression

    Yeast

    Promoter

    GAL 10

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSyn-PSDΔ1,2-FRBx3

    Plasmid

    #225827

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    PSDΔ1,2-FRBx3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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