We narrowed to 27,760 results for: STI;

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1(118-1274)

Plasmid

#221207

Purpose

ScChd1-aa118-1274 chromatin remodeler

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RnBarr2-mVenus

Plasmid

#137793

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

Plasmid

#51473

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6m

Use

AAV

Promoter

hSyn1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT delta CT

Plasmid

#1796

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CT (aa468-673)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
GOMO-GFP

Plasmid

#60975

Purpose

Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

Depositor

Olivier Silvie

Article

Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

Inserts

GFP
mCherry
hDHFR-yFCU

Use

Plasmodium gene targeting

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT DBM (H212R)

Plasmid

#8352

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
L2_UBE2:mT-N7-CsA

Plasmid

#136133

Purpose

Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:mTurq-N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFPV25.1_rpsM_mCerulean3

Plasmid

#124904

Purpose

rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

rpsM promoter and mCerulean3

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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GB4542

Plasmid

#215261

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GB4401

Plasmid

#215252

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YFP-Rac1(N17)

Plasmid

#11395

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 mutated to Asparagine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Expression

Yeast

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP Flag-hRpt6

Plasmid

#19793

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

hRpt6 (PSMC5 Human)

Use

Retroviral

Tags

Flag

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pUCHR-inGFPt

Plasmid

#60237

Purpose

HIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Lentiviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRcCMV Cep164 (Nigg CW325)

Plasmid

#41148

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-HEPN

Plasmid

#75296

Purpose

Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

Insert

Human Sacsin 4441-4579

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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