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pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3-mNG-STOP

Plasmid

#218644

Purpose

Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

mNeonGreen

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean-H2B-6

Plasmid

#55374

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

H2B (H2BC11 Human)

Tags

mCerulean

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

Plasmid

#211491

Purpose

conditional expression of GCaMP8s under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

Plasmid

#211493

Purpose

conditional expression of GCaMP8s under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Sclt1

Plasmid

#128873

Purpose

For mammalian expression of mouse Sclt1

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Sclt1 (Sclt1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pBABE-puro-NLS-LSSmKate1

Plasmid

#31873

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate1

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pKSB-611U6-sgRNA3 AGAG AACA

Plasmid

#173673

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-AGT

Plasmid

#184598

Purpose

For expression of a fluorescent sensor for angiotensin II in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AGT

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-sgControl_dual-MTS

Plasmid

#162765

Purpose

Expressing sgControl_dual-MTS

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgControl_dual-MTS

Expression

Mammalian

Promoter

U6

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR29_00091361

Plasmid

#121209

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR29_00091361

Use

Golden gate

Mutation

Magnaporthe grisea

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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CD3delta-CFP

Plasmid

#128154

Purpose

Expression of CD3 delta

Depositor

Jennifer Lippincott-Schwartz

Article

Lorenz et al Nat Methods. 2006 Mar;3(3):205-10. doi:

Insert

CD3 Delta (Cd3d Mouse)

Expression

Mammalian

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-mcpGFP

Plasmid

#47442

Purpose

contains modified circularly permutated EGFP (mcpGFP)

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

mcpGFP

Expression

Bacterial

Mutation

M154K, V164A, S176G, D181Y, T204V, A207K, V190I

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pCF730

Plasmid

#121668

Purpose

EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE 608 : pHYG_GFP

Plasmid

#92089

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK003-2-pENO1

Plasmid

#84962

Purpose

Type 2, pENO1, Enolase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Enolase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (2350 g to c) and BsmBI site re…

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y861F

Plasmid

#50508

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y861F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP

Plasmid

#179644

Purpose

AND gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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