We narrowed to 65,936 results for: des.1

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-diLACCO1

Plasmid

#208028

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-mCherry-TOSI

Plasmid

#172496

Purpose

mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

PDCD4 (Pdcd4 Mouse)

Use

Retroviral

Tags

Mcherry

Expression

Mammalian

Promoter

MLV LTR

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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BLADE-182

Plasmid

#134914

Purpose

sgRNA targeting GFP to be used in nanoblade system

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GFP

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1)

Plasmid

#191341

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Expression

Mammalian

Promoter

TRE3GV

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC7A1

Plasmid

#131925

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A1 (SLC7A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pFUW-tetO- FOS

Plasmid

#125598

Purpose

doxycycline-inducible expression of human FOS in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Fos proto-oncogene, AP-1 transcription factor subunit (FOS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD WT TET1

Plasmid

#124082

Purpose

Expression of wild-type catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 catalytic domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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p202 Dync1li1 wt

Plasmid

#26450

Depositor

Elizabeth Fisher

Insert

cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCI-OR1A1

Plasmid

#22319

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR1A1 (OR1A1 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Empty Vector-IRES-Puro

Plasmid

#120568

Purpose

For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Type

Empty backbone

Use

Lentiviral

Tags

Flag-HA-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-Kan

Plasmid

#44955

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Expression

Yeast

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_ETS1

Plasmid

#167700

Purpose

Expresses ETS1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETS1 (ETS1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSiMPlc_N + pSiMPlc_C

Plasmid

#134312

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

cmR_gp41-1 N-intein + cmR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-TDsmURFP-RBS-HO-1

Plasmid

#80342

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pRS40P

Plasmid

#35481

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pRS40B

Plasmid

#35478

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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