We narrowed to 24,948 results for: ina
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Plasmid#10739DepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pOPINB-M1-di-Ub(G76V)-HOIL-1
Plasmid#229539PurposeConstitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coliDepositorTags6xHis-3CExpressionBacterialMutationChanged Gly 76 to Val and changed Gly 76 to ValPromoterT7Available sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-AtUBC8-S-AtUBA1-S
Plasmid#222714PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cellsDepositorAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA5-FRT/TO-FLAG-TONSL
Plasmid#221011Purposemammalian expression vector of Flag tagged TONSL siRNA resistantDepositorInsertTONSL (TONSL Human)
TagsFlagExpressionMammalianMutationsilent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…PromoterCMVAvailable sinceJune 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp62 (NUP62 Human)
TagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mcherry p62 delta UBA silent
Plasmid#187984Purposemcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp62 (NUP62 Human)
TagsmCherryExpressionMammalianMutationLacking UBA domain (aa389-434) but it has the sil…Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-pmEMBer
Plasmid#174441PurposePlasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpmEMBer
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG
Plasmid#176060PurposeExpresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
Tags3xFLAGExpressionMammalianMutationK164A and H165APromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…ExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3xFLAG CMV14 / BRAF-G469A
Plasmid#131711PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Zeo p53DD
Plasmid#136534PurposeLentiviral expression vector for an inducible p53DDDepositorInsertp53DD (Trp53 Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…ExpressionMammalianMutationThis construct was created from the pBabe p53DD (…Available sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
Plasmid#135176PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorInsertshChR2
TRE3G
UseAAV and Cre/LoxTagsEYFP (C terminal on insert)ExpressionMammalianMutationE123T and T159CPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113700PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Hygro(-)-B2-TS3
Plasmid#104353PurposeExpresses human beta 2 integrin tension sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman integrin b2 with tension sensor (ITGB2 Human)
ExpressionMammalianMutationAA1-733 ITGB2 presentPromoterCMVAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP
Plasmid#91882PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting Suz12 (Suz12 Mouse)
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN-SOD1
Plasmid#89757Purposeexpresses human SOD1 fused to the N-terminal part of VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOD1 (SOD1 Human)
TagsN-terminal part of VenusExpressionMammalianPromoterCMVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
Plasmid#74505PurposeOverexpression of CSK SH3-SH2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCSK (CSK Human)
TagsFLAGExpressionMammalianMutationSH3-SH2 domainsPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1 1366-1437
Plasmid#71118Purposemammalian expession of mCherry-LacR-BRCA1 1366-1437DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRCA1 (BRCA1 Human)
TagsmCherryExpressionMammalianMutationcontains amino acids 1366-1437 of BRCA1PromoterCMV/TOAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S285A/T289A-3xFLAG
Plasmid#69841PurposeThis plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLK4 (PLK4 Human)
Tags3xFLAG tag and EGFPExpressionMammalianMutationS285A/T289APromoterCMVAvailable sinceDec. 3, 2015AvailabilityAcademic Institutions and Nonprofits only