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pCMV_RTN3E-mScarlet3

Plasmid

#216255

Purpose

mammalian expression vector for RTN3E-mScarlet3

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon 3 isoform e (RTN3 Human)

Tags

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

Plasmid

#215014

Purpose

Express an autophagy (LC3) reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

MAP1LC3B (Map1lc3b Rat)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NLS (Nucleoplasmin)

Plasmid

#220342

Purpose

Mammalian expression vector BS2 in nucleus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NLS (Nucleus localization sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP3

Plasmid

#220070

Purpose

overexpresses ORP3 with a neonGreen Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL3 (OSBPL3 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6/7FTrp)RS

Plasmid

#207621

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-SpEndoH

Plasmid

#218196

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

Depositor

Florence Pojer

Insert

EndoH

Tags

tufA-His-CBD

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 45-313

Promoter

T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo

Plasmid

#209636

Purpose

Expression miniTurbo in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightG

Plasmid

#217656

Purpose

Expresses norepinephrine indicator nLightG under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightG

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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B2380_BTB-IDR

Plasmid

#215098

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

Plasmid

#211840

Purpose

Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA and mCherry

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET28sumo-MDA5-Full-length

Plasmid

#210826

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

Insert

MDA5 (IFIH1) Full Length Protein (IFIH1 Human)

Expression

Bacterial

Mutation

NA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCas3-Km

Plasmid

#207999

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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enz.099-PKA

Plasmid

#154048

Purpose

Expresses PKA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CibN

Plasmid

#209671

Purpose

Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Split-TurboID-N and pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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