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pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

Plasmid

#201686

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pB-TAG-HES7

Plasmid

#130935

Purpose

PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
AAV-SYN_mMaroon-KIX-mMaroon

Plasmid

#137009

Purpose

AAV backbone, mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mMaroon

Expression

Mammalian

Promoter

hSynapsin

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-Tensin1-N-18

Plasmid

#56175

Purpose

Localization: Focal Adhesions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tensin1 (TNS1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pX330 NL-FKBP-Cas9

Plasmid

#162723

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-shNCLX-2

Plasmid

#181871

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-shNCLX-1

Plasmid

#181870

Purpose

Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

solute carrier 8 family member B1 (Slc8b1 Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_2

Plasmid

#160764

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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ROCK2 gRNA (BRDN0001147800)

Plasmid

#76349

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK2 (ROCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#163698

Purpose

AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

Depositor

Gina Turrigiano

Article

Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

Insert

CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mammalian

Promoter

CaMKII fragment

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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attB53_SNCB+_attB53

Plasmid

#183614

Purpose

RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

LaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP

Use

Rmce

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBM-mut

Plasmid

#187150

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UIM-mut

Plasmid

#187148

Purpose

mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

A1376G/S1382A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA-mut

Plasmid

#187146

Purpose

mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

M1328A/F1330A

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX330 N-FKBP-Cas9

Plasmid

#162727

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ265G2

Plasmid

#164723

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-ziSpyMac(D10A)-UGI)

Use

CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

Expression

Plant

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124543

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55G, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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MDH1-shSHP2-3-H2Kb2

Plasmid

#17854

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

shSHP2 (PTPN11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available Since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

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