We narrowed to 18,247 results for: multi
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Plasmid#213292PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLRD11
Plasmid#208263PurposeProduction of sclareol in E. coli MEV20DepositorInsertstHMGR
abCAS
ssSS
ExpressionBacterialMutationabCAS(D537A)Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-320
Plasmid#195448PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF3DepositorInsert8X ZF3 BS - minCMV - mCherry
UseLentiviralExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-321
Plasmid#195449PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert8X ZF10 BS - minCMV - mCherry
UseLentiviralExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10p CAS9 Gal1/10p I-SceI
Plasmid#182519PurposeGalactose-Induced Expression vector for Cas9 and I-SceI.DepositorInsertsI-SceI
Cas9
ExpressionYeastAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-lacZ-stop-L2
Plasmid#186359PurposeEntry clone with ORF encoding lacZ flanked by Gateway recombination sequences.DepositorPublicationInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of lacz reporterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.419
Plasmid#187208PurposeExpresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2DepositorInsertsRetron Eco4 ncRNA
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE-->2xNLS_IFP_PGK_PuroR
Plasmid#162238PurposeLentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cellsDepositorInsertNLS IFP
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAPtag-LacI
Plasmid#183922PurposeLac repressor labeled with SNAPtag; can be used to label lacO sitesDepositorAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C/S-ires-mCD8
Plasmid#183719PurposeExpresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.DepositorInsertSHP-1
UseRetroviralTagsmycMutationC453SAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F2
Plasmid#184167PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmClover3
bsd
UseCRISPRAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMC_mClover3_BSDminus_F1
Plasmid#184166PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmClover3
bsd
UseCRISPRAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-MmAcKRS1-PylTUUA
Plasmid#182537PurposeN‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppressionDepositorInsertPylTUUA
ExpressionBacterialPromoterProKAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL728
Plasmid#169152PurposeEncodes OptoQ-AMP6DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG552-MAP4-redFAST
Plasmid#160367PurposeExpresses MAP4-redFAST in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAP4-redFAST
ExpressionMammalianAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Tt
Plasmid#154124PurposeExpresses wt Ribo-T rRNA and a set of tRNAs in bacterial cellsDepositorInsertsengineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
UseSynthetic BiologyExpressionBacterialPromoterPtac and pLAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only