We narrowed to 21,605 results for: ESC;

1. pDGE10

   Plasmid

   #79466

   Purpose

   sgRNA shuttle vector; module 4

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYCDuet_D27NDHFR_WTTS

   Plasmid

   #91244

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D27N

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLOT-syb-sp-YFP1-10

   Plasmid

   #73700

   Purpose

   Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spYFP1–10 chimera
   

   Use

   Lexaop reporter vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mEos2-Calnexin-N-14

   Plasmid

   #57352

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pGas-miRFP670nano3

   Plasmid

   #184672

   Purpose

   G protein alpha-subunit internally tagged with miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   G protein alpha-s-miRFP670nano3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFPL-Yh2

   Plasmid

   #61650

   Purpose

   Gateway vector for generating yellow fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pPaxillin-TagRFP675-N1

    Plasmid

    #44276

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    chicken paxillin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pmCitrine_N1

    Plasmid

    #92420

    Purpose

    Empty backbone for C-terminal fusion of mCitrine

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM6-At4CL-PhCHS-CmCHI

    Plasmid

    #73393

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ManII-SBP-mCherry_puromycin

    Plasmid

    #65261

    Purpose

    ManII reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated Mannosidae II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEBTet-p52-HDM2_sensor

    Plasmid

    #127184

    Purpose

    Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KG#59

    Plasmid

    #110929

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZH509

    Plasmid

    #102664

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-DiB2

    Plasmid

    #168801

    Purpose

    Fluorogen activating protein (FAP) DiB2

    Depositor

    Alexander Mishin

    Article

    Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

    Insert

    Fluorogen activating protein DiB2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-REPORT(EF1a no GFP) mnuctdTomato

    Plasmid

    #172342

    Purpose

    mnuctdTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR4_EWSR1

    Plasmid

    #26367

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only