We narrowed to 9,183 results for: Pol

1. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

   Plasmid

   #99690

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_128

   Plasmid

   #160651

   Purpose

   Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e3 (APOE Human)
   

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e3 allele

   Promoter

   pZ estradiol inducible promoter

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDHa_mDDX5_FL_wt

   Plasmid

   #88869

   Purpose

   constitutive expression of Ha-DDX5 in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (Ddx5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS_107

   Plasmid

   #160650

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.RBBP4

   Plasmid

   #125164

   Purpose

   Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Histone-binding protein RBBP4 (RBBP4 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDHa_mDDX5_FL_K144N

   Plasmid

   #88870

   Purpose

   constitutive expression of Ha-DDX5 K144N in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (Ddx5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K144N

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGS_129

   Plasmid

   #160652

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   pZ estradiol inducible promoter

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMVtrunc sfCherry2ΔC4-Lifeact

   Plasmid

   #231557

   Purpose

   Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Tags

   sfCherry2

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Donor_ANKRD1_KO_Puro

    Plasmid

    #186667

    Purpose

    Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO Puromycin (ANKRD1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB1.HMBP.PrS.AEBP2

    Plasmid

    #125165

    Purpose

    Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Zinc finger protein AEBP2 (AEBP2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Donor_YTHDF2_KO_Hygro

    Plasmid

    #186671

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Hygromycin (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Donor_YTHDF2_KO_Puro

    Plasmid

    #186670

    Purpose

    Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO Puromycin (YTHDF2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-pLKO.neo_shGFP

    Plasmid

    #110470

    Purpose

    Control, silence GFP gene, doxycycline inducible, neomycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-CRY2-INPP5E

    Plasmid

    #79561

    Purpose

    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    C-terminal region, aa 214_644, C641A mutation to …

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-SM

    Plasmid

    #136578

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-E2A-cMyc (Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PTRF/Cavin1_L (OZ513)

    Plasmid

    #27188

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pLIB_GST-nsp13 (SARS-CoV-2)

    Plasmid

    #169190

    Purpose

    Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    GST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    GST

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LIC4B-His-TEV-TAK1-TAB1

    Plasmid

    #154874

    Purpose

    Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

    Depositor

    Kenneth Westover

    Article

    Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

    Insert

    TAK1-TAB1 (TAB1 Human)
    

    Tags

    LIC TEV His

    Expression

    Insect

    Mutation

    Codon optimized

    Promoter

    polyhedrin

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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