We narrowed to 63,772 results for: cell

1. pCW57.1 _3xHA-Ago2_CATmut

   Plasmid

   #73540

   Purpose

   Inducible lentiviral expression of Ago2_CATmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Catalytic mutant PIWI domain D670A

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB-hCRY1-Halo-CD4-bla

   Plasmid

   #179445

   Purpose

   Donor vector to knock in Halotag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   HaloTag (Halo7 Synthetic)
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-E2F1

   Plasmid

   #37141

   Depositor

   Robert Duronio

   Article

   Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

   Insert

   E2F1 (E2f1 Fly)
   

   Use

   Gateway

   Available Since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

   Plasmid

   #26797

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
   

   Expression

   Mammalian

   Mutation

   Human 5HT4B receptor with D100A mutation, generat…

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-tagBFP-hTRF1

   Plasmid

   #103801

   Purpose

   BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hTRF1 (CIB1, TERF1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1062

   Plasmid

   #29011

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple155-Cre/EGFP-NLS
   

   Use

   High copy number homologous recombination plasmid…

   Expression

   Mammalian

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-RAB10 K154A

   Plasmid

   #130886

   Purpose

   Expresses human RAB10 K154A mutant with N-terminal GFP tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   RAB10 (RAB10 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K154A

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EFS-hTSC1-T2A-Bsd

   Plasmid

   #193199

   Purpose

   Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EPHB4-2A-EGFP

    Plasmid

    #139968

    Purpose

    Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    Human EPHB4 knockin homology arms (EPHB4 Human)
    

    Expression

    Mammalian

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-CES2A-ΔC-S227A

    Plasmid

    #200556

    Purpose

    Mammalian expression of secreted mouse CES2A S227A mutant

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST40-CES2A-ΔC

    Plasmid

    #200554

    Purpose

    Mammalian expression of secreted mouse CES2A

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Tbg-CES2A-ΔC

    Plasmid

    #200558

    Purpose

    Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Promoter

    TBG promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-SEMA3C-COMP5AP-AviTag-9xHis

    Plasmid

    #157525

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3C (SEMA3C Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN-CD4

    Plasmid

    #175942

    Purpose

    knockout mouse CD4

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    CD4 gRNA (Cd4 Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Luc-Sox2-3'UTR

    Plasmid

    #63895

    Purpose

    Luciferase reporter of mouse wildtype Sox2 3'UTR

    Depositor

    Lin He

    Article

    Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Expression

    Mammalian

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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