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pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCB’

Plasmid

#162703

Purpose

pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

5513T>G (tetR E37A); 7758G>T (second tet op…

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Rictor-Myc

Plasmid

#157931

Purpose

Over expression of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor (Rictor Mouse)

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

Ubiquitinc Promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hEzh2-IRES-Hyg

Plasmid

#122375

Purpose

Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hEzh2-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R267

Plasmid

#138179

Purpose

Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGY).gp41-1.(SSS)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin1

Plasmid

#108888

Purpose

Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin1 (altered codon usage) (CAVIN1 Synthetic, Human)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

Plasmid

#71217

Purpose

dCas9 fused to human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B catalytic domain

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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mVenusN-Cavin1

Plasmid

#108887

Purpose

Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

caveolae associated protein 1 (CAVIN1 Synthetic, Human)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-mCherry-iLIDx6

Plasmid

#103782

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-mCherry-iLIDx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Flag-hSPDEF WT

Plasmid

#100873

Purpose

Lentiviral expression of human SPDEF

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PAT1

Plasmid

#88856

Purpose

myc-TAG overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

SLC36A1 (SLC36A1 Human)

Tags

myc

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-Tet1 promoter B

Plasmid

#63882

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment B

Use

Luciferase

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-MAT2B

Plasmid

#34795

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

methionine adenosyltransferase II, beta (MAT2B Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1

Plasmid

#35376

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-365

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pUASTP2.1

Plasmid

#13842

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

mini-white (w Fly)

Expression

Insect

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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3440 pcDNA3 Bid p22

Plasmid

#8774

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 wt (Bid Mouse)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only