20,081 results

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-MITF

Plasmid

#64888

Purpose

Retroviral expression of murine MITF

Depositor

Ivana de la Serna

Article

de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

Insert

MITF (Mitf Mouse)

Use

Retroviral

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCrispr-V2-mOrange (V2mO)

Plasmid

#140206

Purpose

Lenti Crispr/Cas9 with mOrange fluorescent marker

Depositor

Jaffer Ajani

Article

Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pELF1a

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-WT

Plasmid

#82033

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-YY1_1

Plasmid

#36357

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

YY1 (YY1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
NTSR1-Tango

Plasmid

#66457

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

NTSR1 (NTSR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Puro empty (w610-1)

Plasmid

#20953

Purpose

3rd gen lentiviral empty control vector, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Tags

No tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBbB8k-GFP

Plasmid

#35363

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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TNFR2-GFP

Plasmid

#111207

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TNFRSF1B (TNFRSF1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

S175P, D306G

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-ProRS-His

Plasmid

#124117

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Prolyl-tRNA ligase (proS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pHoss1

Plasmid

#63158

Purpose

Suicide plasmid for efficient gene mutation in Gram-positive bacteria

Depositor

Attila Karsi

Article

Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

Insert

anti-secY-Pxyl/tetO-tetR

Use

Suicide plasmid for gram-positive bacteria

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hygro EYFP H148Q/I152L

Plasmid

#25874

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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