We narrowed to 12,197 results for: 112

1. CDA1-BE3

   Plasmid

   #100804

   Purpose

   Expresses CDA1-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   CDA1-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-1 3xFlag-ERK2

   Plasmid

   #47573

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged ERK2

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   ERK2 (Mapk1 Rat)
   

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

4. plenti hSyn AldoA

   Plasmid

   #220916

   Purpose

   Neuronal specific expression of AldoA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   AldoA (ALDOA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR RPS27A-3xFlag_IRES-GFP

   Plasmid

   #198390

   Purpose

   RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. cpGFP-Adhiron_flex

   Plasmid

   #165044

   Purpose

   Flexible linker control for POLArISact expression in mammalian cells

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   cpGFP-Adhiron (connected with a flexible linker)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mPCSK8

   Plasmid

   #122677

   Purpose

   Expresses mouse PCSK8 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv1_UQCRC2

   Plasmid

   #177982

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-AD8-QES.i08.c04

   Plasmid

   #123259

   Purpose

   Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations P124D, …

   Promoter

   CMV

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA_DNMT3a-T1

    Plasmid

    #41821

    Purpose

    Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_DNMT3a-T1 (DNMT3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLHCX-Flag-mPKM2(C358S)

    Plasmid

    #42513

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Cys358->Ser
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    Changed Cysteine 358 to Serine

    Promoter

    CMV

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. MiniCoopR U6:gRNA p53, mitfa:Cas9

    Plasmid

    #118841

    Purpose

    Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    tp53 gRNA (tp53 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-51b(+)-Caprola_09

    Plasmid

    #194670

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs_FLAG-GGC1

    Plasmid

    #133258

    Purpose

    Express FLAG-tagged GGC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    GGC1 (GGC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGL1252_TetOFF(Hyg)

    Plasmid

    #118992

    Purpose

    TetOFF (promoter-less) cassette with Hygromycin selection

    Depositor

    Naweed Naqvi

    Article

    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

    Inserts

    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    

    Use

    Fungal expression (in magnaporthe oryzae)

    Mutation

    Codon optimized for Magnaporthe oryzae

    Promoter

    Aspergillus nidulans TrpC promoter, Ustilago mayd…

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCT-GAL93-SFPQ

    Plasmid

    #46320

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    SFPQ (SFPQ Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

    Plasmid

    #42046

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Expression

    Mammalian

    Mutation

    G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs_FLAG-MCART2

    Plasmid

    #133250

    Purpose

    Express FLAG-tagged MCART2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART2 (SLC25A52 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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