We narrowed to 1,203 results for: LIPI

1. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Use

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-hATG2A

   Plasmid

   #114462

   Purpose

   Expresses human ATG2A with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

   Insert

   human ATG2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   P656R (please see depositors comment below)

   Promoter

   CMV

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMP34-mApple

   Plasmid

   #214417

   Purpose

   Peroxisome localization of PMP34 fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIP mScarlet-MOSPD2 WT

   Plasmid

   #186472

   Purpose

   Expression of human MOSPD2 fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-EFIRES-P-GFP(1-10)opti

   Plasmid

   #129416

   Purpose

   Expressing codon-optimized GFP(1-10) fragment in human cells

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   codon-optimized GFP(1-10)
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTSK01K_0G7

   Plasmid

   #177296

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

   Depositor

   Karl-Erich Jaeger

   Article

   Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

   Insert

   PaacC1-aacC1
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-PI35P2

   Plasmid

   #92419

   Purpose

   Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

   Insert

   MCOLN1 (Mcoln1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Synthetic gene, codon optimized for human.

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-2xPH-TAPP1 R211L mutant

   Plasmid

   #161991

   Purpose

   Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R211L

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDC43-FIT2

    Plasmid

    #96993

    Purpose

    Express GFP-tagged mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    E2D (please see depositor comments below)

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLHCX-mPM20D1-flag

    Plasmid

    #84566

    Purpose

    mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

    Depositor

    Bruce Spiegelman

    Article

    Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

    Insert

    peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    Retroviral

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

    Plasmid

    #169079

    Purpose

    Human ATG3

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6X Histidine Tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Sec22b-shR

    Plasmid

    #208358

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations introduced to render shRNA …

    Promoter

    CMV

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mouse Alpha2 delta-2 HA pMT2

    Plasmid

    #58731

    Purpose

    expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-MGAT1-KO

    Plasmid

    #80009

    Purpose

    gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    MGAT1 (MGAT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav3P104L-YFP

    Plasmid

    #68404

    Purpose

    Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells

    Depositor

    Rob Parton

    Article

    Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.

    Insert

    Cav3 (Cav3 Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Proline at position 104 changed to a Leucine

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. M3-PSCFP2

    Plasmid

    #114185

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only