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We narrowed to 1,212 results for: Ncl

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Showing: 881 - 900 of 1212 results

  1. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  4. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  8. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  14. pENTR-AtMIR390a-B/c

    Plasmid
    #51778
    Purpose
    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Gateway entry vector
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51778-plasmid-map-sequence-id-79411

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  18. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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  19. PBE-NG

    Plasmid
    #229045
    Purpose
    Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.
    Depositor
    Huawei Zhang
    Article
    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229045-plasmid-map-sequence-id-459812

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  20. pIL6-Nluc-hbb

    Plasmid
    #239843
    Purpose
    Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
    Use
    Luciferase
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239843-plasmid-map-sequence-id-477827

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Showing: 881 - 900 of 1212 results