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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M

Plasmid

#146948

Purpose

Insect Expression of DmAGO1-del835-886-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GGSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M

Plasmid

#146949

Purpose

Insect Expression of DmAGO1-del835-886-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pB-TR-TTHA

Plasmid

#232479

Purpose

Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Article

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Thermus thermophilus HB8

Use

Transposon system

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Promoter

CMV-TetO

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHes1(467 RBPj(-))-luc

Plasmid

#43805

Depositor

Ryoichiro Kageyama

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter (-467 to +46), …

Promoter

Hes1 promoter fragment (-467 to +46) (RBPj-)

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only
pMAPLe4 PCMTD1

Plasmid

#246503

Purpose

Recombinant protein expression of PCMTD1

Depositor

Steven Clarke

Article

Warmack et al Biochemistry. 2022 May 17;61(10):879-894

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdhV5-5BoxB_X

Plasmid

#147910

Purpose

Insect Expression of DmAdh-5BoxB

Depositor

Elisa Izaurralde

Insert

DmAdh-5BoxB (Adh Fly)

Expression

Insect

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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tout velou (ttv)

Plasmid

#37401

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

tout-velu (ttv Fly)

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi-NF

Plasmid

#86100

Purpose

Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A and S71T, G73A

Promoter

TlpA and TlpA, LacI

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
orthodenticle

Plasmid

#37285

Depositor

Norbert Perrimon

Article

Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

Insert

orthodenticle (oc Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
UAS-DExt (ttv)

Plasmid

#41180

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

dext (ttv Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
UAS-zw4 (egghead) ORF

Plasmid

#38764

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

zw4 (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCaShs-egghead (zw4)

Plasmid

#37400

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pETSUMO-TtAgo

Plasmid

#210191

Purpose

Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H08

Plasmid

#70996

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Nap1 (Nap1 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2B10

Plasmid

#70926

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Keap1 (Keap1 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only