We narrowed to 7,120 results for: RAP
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Plasmid#226101PurposeHRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cellsDepositorInsertHRI (EIF2AK1 Human)
UseTagsGFPExpressionMammalianMutationSIFI degron helices 1 & 2 deleted (Δ61-112)PromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-N-HRI(1-138)-GFP-IRES-mCherry
Plasmid#226100PurposeHRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cellsDepositorInsertHRI (EIF2AK1 Human)
UseTagsGFPExpressionMammalianMutationcontains only N-HRI (aa 1-138)PromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRE3G_hRTN3E-Halo
Plasmid#216252PurposeTet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vectorDepositorInsertreticulon-3 isoform e (RTN3 Human)
UseLentiviralTagsHaloTagExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c001
Plasmid#175466PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0015
Plasmid#141475PurposeLentiviral vector for overexpressing the DRAP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDRAP1 (DRAP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc-his-BACH1 P47A
Plasmid#17644DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationTo generate the tumor-associated mutant, the prol…PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 ElonginB, ElonginC
Plasmid#230999PurposeDual expression vector containing mouse ElonginB and ElonginC for E.coli protein purificationDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-VRQR (pBM1192)
Plasmid#140254PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFPDepositorInserteUNG-BE4max(R33A)_VRQR-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Ruby3-Rab7A
Plasmid#135651PurposeRab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.DepositorInsertRab7A (RAB7A Human)
UseTagsRuby3ExpressionMammalianMutationPromoterL30Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_GST-GBRP
Plasmid#223728PurposeExpression of recombinant protein for purificationDepositorInsertGABARAP (GABARAP Human)
UseTagsGSTExpressionBacterialMutationaa 2-116 only, with Glycine exposedPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-MCRS1
Plasmid#231241PurposeFlag-tagged wild-type MCRS1 over-expression construct for transient expression in mammalian cellsDepositorInsertMCRS1 (MCRS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-sumo
Plasmid#226107PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assaysDepositorInsertDELE1 (DELE1 Human)
UseTagsSumoExpressionMammalianMutationencodes only residues 142-515 of DELE1 (represent…PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTS-cox8A-GFP-IRES-mCherry
Plasmid#226103PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
UseTagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-ATF4uORF1-2-GFP-IRES-mCherry
Plasmid#226105PurposeATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)DepositorInsertatf4 (ATF4 Human)
UseTagsGFPExpressionMammalianMutationcontains uORF1-2 of ATF4, whose stability can ser…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Flag-Apex-Rab7A
Plasmid#135652PurposeRab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.DepositorInsertRab7A (RAB7A Human)
UseTagsApex2 and FLagExpressionMammalianMutationPromoterL30Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry
Plasmid#231008PurposeProtein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.DepositorInsertCDK5R1 aa283-307 (CDK5R1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC-GFP-IRES-mCherry
Plasmid#231232PurposeMYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only