We narrowed to 13,035 results for: SHI

1. pCMV-dCas12a-A3G

   Plasmid

   #193643

   Purpose

   Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

   Plasmid

   #162615

   Purpose

   Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M337V

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2plus-mVenus1-155-GGGS-TDP-43

   Plasmid

   #162611

   Purpose

   Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-M-Flag-BAP1-G185R

   Plasmid

   #125849

   Purpose

   express mutant BAP1-G185R

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   G185R

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

   Plasmid

   #194755

   Purpose

   Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Insert

   PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97309

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

   Plasmid

   #75126

   Purpose

   Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   KMT6 (EZH2 Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   SET domain replaced with the SET domain of EZH1

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLVX-M-Flag-BAP1-E31A

   Plasmid

   #125843

   Purpose

   express mutant BAP1-E31A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   E31A

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-M-Flag-BAP1-S10T

    Plasmid

    #125842

    Purpose

    express mutant BAP1-S10T

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    S10T

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-M-Flag-BAP1-L49V

    Plasmid

    #125844

    Purpose

    express mutant BAP1-L49V

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    L49V

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197025

    Purpose

    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized RhCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-M-Flag-BAP1-F170C

    Plasmid

    #125848

    Purpose

    express mutant BAP1-F170C

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    F170C

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-M-Flag-BAP1-G109V

    Plasmid

    #125846

    Purpose

    express mutant BAP1-G109V

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    G109V

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-crRNA(TARDBP)

    Plasmid

    #109323

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TARDBP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 7AA-aSyn-pRK172

    Plasmid

    #236197

    Purpose

    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.

    Depositor

    Yang Yang, Sjors Scheres

    Article

    Yang et al Acta Neuropathol. 2023 May;145(5):561-57

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insertion os MAAAEKT at residue 22

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-dCas12a-A3A

    Plasmid

    #193638

    Purpose

    Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6-sgRNA-hcr3

    Plasmid

    #193660

    Purpose

    Expression of tandem pre-sgRNA array hcr3 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge