Addgene

We narrowed to 2,312 results for: crispr system

Showing: 881 - 900 of 2312 results

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pSIR-neo

Plasmid

#51128

Purpose

Self-inactivating retrovirus vector containing a neomycin-resistance gene

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSIR-hCD2

Plasmid

#51143

Purpose

Self-inactivating retrovirus vector containing the human CD2 gene

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL4723-P1

Plasmid

#191786

Purpose

Level P Golden gate accepter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMK438 (Stag-3FLAG-P2A-Hygro)

Plasmid

#168233

Purpose

C-terminal tagging with Stag-3FLAG

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

Stag-3FLAG-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC637

Plasmid

#182956

Purpose

AD-SYNZIP: αNTD (P. aeruginosa) – SYNZIP17

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Insert

AlphaNTD( P. aeruginosa) - SynZip17

Use

Tags

SynZip17

Expression

Bacterial

Mutation

Promoter

J23106

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMK439 (Stag-3FLAG-P2A-BSD)

Plasmid

#168234

Purpose

C-terminal tagging with Stag-3FLAG

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

Stag-3FLAG-P2A-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pR6K-GFP-CASTIF

Plasmid

#199653

Purpose

R6K plasmid encoding a type I-F CAST but no CRISPR array

Depositor

IIya Finkelstein

Insert

CAST I-F systems

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119 promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

Plasmid

#178883

Purpose

Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

U6-Nla repeat-BbsI-Nla repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

Plasmid

#132401

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

Plasmid

#132408

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLbaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R600A; H605A; R1243A; H1248A

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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