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Plasmid#44187DepositorInsertHistone tagged GFP, T2A-TVA-E2A-B19G
UseAAV; Adeno-associated vectorTagsGFPExpressionMutationPromoterEF1aAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_HD (SPC right)
Plasmid#126695Purposeright TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC right
UseTALENTagsNLS, truncated N term and TAL, FokIExpressionMammalianMutationR451P in SPCPromoterEF1Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_NN (SPC left)
Plasmid#126694Purposeleft TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC left
UseTALENTags0.5 repeat NN, TAL, FokI and NLS, truncated N ter…ExpressionMammalianMutationPromoterEF1Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
Plasmid#98973PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 2 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen
Plasmid#184695PurposeLentiviral transfer plasmid for expression of GFP ArgSenDepositorInsertGFP ArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.28.BB.3z
Plasmid#200676PurposeModular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a CHR2 mCherry WPRE
Plasmid#129432Purposeexpression of CHR2 mCherry from EF1a promoterDepositorInsertCHR2 mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-Fkbp1x 2A Hygro
Plasmid#102806PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion proteinDepositorInsertMS2-Fkbp
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-puro
Plasmid#167819Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV Ef1a-mRuby3-WPRE
Plasmid#135427PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP RanSen
Plasmid#184705PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4(TA) + PGK-blast
Plasmid#167827PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-tdTomato
Plasmid#122099PurposeAAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7-linker-mAID-W
Plasmid#159294PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoterDepositorInsertKAT7 (KAT7 Human)
UseLentiviralTagsmAID (auxin-inducible degron)-taggedExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10)Promoterhuman EF1a promoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationC124SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP-WPRE
Plasmid#44352DepositorInsertEF1a-GFP
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2526-3969)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-3
Plasmid#24415DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)-TTN
Plasmid#172327PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only