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FLAG-NLRC5

Plasmid

#37521

Depositor

Thomas Kufer

Publication

Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

Insert

NLRC5 (NLRC5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pP2A-mCherry-N1

Plasmid

#84329

Purpose

Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

Depositor

Dorus Gadella

Publication

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

P2A-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Publication

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLX304-FOS-V5

Plasmid

#59140

Purpose

wild-type FOS with C-terminal V5 tag expressed from pLX vector

Depositor

William Hahn

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

FOS (FOS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

Plasmid

#137163

Purpose

Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-ChR2-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Publication

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Publication

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSL0283 (pTnsABC)

Plasmid

#130633

Purpose

Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

Depositor

Samuel H. Sternberg

Publication

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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L4437 x6TRE (cJun and AP-1) Ben DsRed-Express2 in TUPV1

Plasmid

#244197

Purpose

Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (AP-1 binding sites)

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

cJun responsive promoter (6 AP-1 binding sites + YB_TATA), dsRedExpress2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNIC-CTHF

Plasmid

#26105

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

TEV - His6 - Flag

Expression

Bacterial

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Publication

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJSK664

Plasmid

#205130

Purpose

Expression of GFP-2xPX (from SnxA) in mammalian cells

Depositor

Jason King

Publication

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

EGFP

Expression

Mammalian

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Publication

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen

Plasmid

#99134

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Publication

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Publication

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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