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pBOB-Adcy3-HA

Plasmid

#245988

Purpose

Express HA C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-L278H-3xFlag

Plasmid

#245990

Purpose

Express 3xFlag C-terminal tagged mouse ADCY3-L278H mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L278H

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-3xFlag

Plasmid

#245989

Purpose

Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45

Plasmid

#245982

Purpose

Express wild-type mouse GPR45 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-Y287C

Plasmid

#245983

Purpose

Express mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P

Plasmid

#245984

Purpose

Express mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1as-ZIM3-Cas9-P2A-GFP

Plasmid

#239603

Purpose

Constitutive active CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186WT-HA

Plasmid

#239781

Purpose

Expresses WT rat NF186 and with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

Plasmid

#200845

Purpose

Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP122: pHelper(CyCAST)_Shuffled-spacer

Plasmid

#200841

Purpose

Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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MFE23-ZZ

Plasmid

#223553

Purpose

MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET302-MFE23-ZZ

Tags

HA tag and HIS tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-wt

Plasmid

#201553

Purpose

Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Expression

Bacterial

Mutation

Codon-optimized for E. coli

Promoter

RBS

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-IEo

Plasmid

#201992

Purpose

AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Depositor

Wei Xu

Article

Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

Insert

Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Use

AAV

Promoter

Tight TRE

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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