We narrowed to 1,221 results for: sumo

1. RBD#3L

   Plasmid

   #193020

   Purpose

   hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

   Depositor

   Xue Gao

   Article

   Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

   Insert

   Cas13a
   

   Use

   Tags

   hnRNP A1 RRM2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMT3-FBF2

   Plasmid

   #166972

   Purpose

   Expresses FBF-2 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-His6-Ubc9-C93A

   Plasmid

   #133910

   Purpose

   Bacterial expression of His6-Ubc9-C93A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 93 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 IST1 (1-366)

   Plasmid

   #193039

   Purpose

   Bacterial expression plasmid for full-length IST1 (1-366).

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 CAPN7 (1-75)

   Plasmid

   #193031

   Purpose

   Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 1-75

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CAPN7 (81-165)

   Plasmid

   #193032

   Purpose

   Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 81-165

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CHMP7 366-453

   Plasmid

   #104598

   Purpose

   Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP7 residues 366-453 (CHMP7 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTVKRSF225

   Plasmid

   #135005

   Purpose

   MusF2 prenyltransferases from Nmu. PCC 7906

   Depositor

   Hideo Iwai

   Article

   Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

   Insert

   MusF2 prenyltransferaseM
   

   Use

   Tags

   SUMO fusion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBHRSF227

    Plasmid

    #135006

    Purpose

    MusF1 prenyltransferase from Nostoc sp. UHCC 0398

    Depositor

    Hideo Iwai

    Article

    Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

    Insert

    MusF1 prenyltransferase
    

    Use

    Tags

    SUMO fusion

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF-rM1PYK

    Plasmid

    #220232

    Purpose

    Inducible expression of rabbit muscle pyruvate kinase isoform 1

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Rabbit muscle pyruvate kinase (PKM )
    

    Use

    Tags

    6xHis-MBP-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHTSHP-Smt-IIShhN

    Plasmid

    #121135

    Purpose

    Expresses N-terminal SUMO tagged II-ShhN in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, MBP, and Smt tag

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-195, with two extra i…

    Promoter

    tac

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 huPQBP1

    Plasmid

    #217449

    Purpose

    Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 IST1 L328D, L355A (1-366)

    Plasmid

    #193040

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, L355A

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CAPN7 V18D,F98D (1-165)

    Plasmid

    #193034

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    V18D, F98D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 IST1, residues 316-366, C-Cys

    Plasmid

    #191234

    Purpose

    Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 316-366, contains nonnative C-terminus f…

    Promoter

    T7

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge