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pcDNA3-IKZF2-V2-V5

Plasmid

#107391

Purpose

Mammalian expression of IKZF2-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2-V2 (IKZF2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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CCT8_pCSdest

Plasmid

#53795

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CCT8 (CCT8 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MBP-TSF-TEVcs-ULK1K46I

Plasmid

#171417

Purpose

For the purifcation of human ULK1 kinase dead complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Expression

Mammalian

Mutation

kinase dead

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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NSP6-FR-2Strep

Plasmid

#210317

Purpose

Expresses SARS-CoV-2 NSP6 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

Plasmid

#194685

Purpose

CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Snail 6SA

Plasmid

#16228

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6SA: S97A, S101A, S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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GST-Delta23C11orf83

Plasmid

#65849

Purpose

bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of the 23 first aa (TM)

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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ZJOM43

Plasmid

#133652

Purpose

Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-VPS4-DuDre (VPS4 Budding Yeast)

Tags

DuDre

Expression

Yeast

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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SSTR3-DuET

Plasmid

#213378

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

SSTR3 (SSTR3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Mouse, Human)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp140-TM

Plasmid

#123251

Purpose

Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Tags

CD5 leader peptide and Linker (with 6his tag) and…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Calnexin-N-14

Plasmid

#158004

Purpose

Visualization of ER in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Calnexin (CANX Human)

Tags

mGold

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript II KS(+) EACMCV DNA-A 1.6mer

Plasmid

#159136

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

EACMCV DNA-A 1.6mer

Use

Infectious clone for bombarding plants

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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EFS-Vector

Plasmid

#109395

Purpose

AAV-CRISPR knockout plasmid for direct in vivo genome editing

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Promoter

EFs-NS

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mCerulean3

Plasmid

#127592

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Myb

Plasmid

#139826

Purpose

doxycycline-inducible expression of mouse Myb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Myb (Myb Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

P94H and Q619R- Please see depositor comment belo…

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI

Plasmid

#221219

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

Plasmid

#194687

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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