We narrowed to 12,197 results for: 112

1. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-FluoSTEP-JNKAR

   Plasmid

   #181857

   Purpose

   Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-JNKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-FluoSTEP-AktAR

   Plasmid

   #181856

   Purpose

   Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AktAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti hSyn PGK1-HALO

   Plasmid

   #220910

   Purpose

   Neuronal specific expression of the fusion construct PGK1-HALO

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-HALO (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO mouse ULK2 shRNA 93

   Plasmid

   #27635

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse ULK2 shRNA 93
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pETDuet-1 TIM8,13

   Plasmid

   #170282

   Purpose

   Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

   Insert

   yeast Tim8 and Tim13 (TIM8 Budding Yeast)
   

   Tags

   TEV-cleavable N-terminal His tag on Tim13 (on bac…

   Expression

   Bacterial

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs_FLAG-SFXN1

   Plasmid

   #110634

   Purpose

   Retroviral expression of Flag-SFXN1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   SFXN1 (SFXN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. NUDT16L1

    Plasmid

    #155795

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    NUDT16L1 (NUDT16L1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA_DNMT3b

    Plasmid

    #41823

    Purpose

    Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_DNMT3b (DNMT3B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCas3-Sm

    Plasmid

    #208001

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMXs_FLAG-MCART6

    Plasmid

    #133251

    Purpose

    Express FLAG-tagged MCART6 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART6 (SLC25A53 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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