We narrowed to 18,496 results for: Met

1. pAB301-Tier1-PhCMV-rTetR_VPR

   Plasmid

   #169579

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   reverse tetracycline-controlled transactivator
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

   Plasmid

   #162967

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Dual Strep2 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-RB (561-673)

   Plasmid

   #31495

   Depositor

   Julien Sage

   Article

   Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

   Insert

   RB (RB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains amino acids 561-673

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3

   Plasmid

   #112171

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102Y.Y137L
   

   Use

   AAV

   Tags

   mRuby3

   Mutation

   F102Y.Y137L

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH227-Tier1-OTetO7-PCMVmin2-SEAP

   Plasmid

   #169563

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP production
   

   Expression

   Mammalian

   Promoter

   TetO7-PCMVmin

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138739

   Purpose

   Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x0
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-rat FceRI alpha

   Plasmid

   #8373

   Depositor

   Jean Kinet

   Article

   Kinet et al Biochemistry 1987 Jul 28;26(15):4605-10.

   Insert

   rat Fc epsilon RI alpha cDNA (Fcer1a Rat)
   

   Use

   Beta-gal

   Expression

   Bacterial

   Available Since

   June 7, 2005

   Availability

   Academic Institutions and Nonprofits only

8. GluR2_adRNA(2,100,50)-GFP

   Plasmid

   #124622

   Purpose

   Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   GluR2_adRNA(2,100,50)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GluR2_adRNA(0,100,50)-GFP

   Plasmid

   #124623

   Purpose

   Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   GluR2_adRNA(0,100,50)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSuper.Retro.Puro-PKN1 shRNA 4

    Plasmid

    #59978

    Purpose

    Knockdown of PKN1

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    PKN1 shRNA
    

    Use

    Retroviral

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pJP102

    Plasmid

    #176480

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > NatR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJP103

    Plasmid

    #176481

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > HygR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2404-Tier1-PhCMV-NeoR

    Plasmid

    #169496

    Purpose

    Tier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-retro-puro-shNup35-EcoRI

    Plasmid

    #87332

    Purpose

    To express shRNA against human Nup35

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup35
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-RB (654-752)

    Plasmid

    #31496

    Depositor

    Julien Sage

    Article

    Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

    Insert

    RB (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 654-752

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pFOX13-Tier1-PhCMV-tGFP

    Plasmid

    #169510

    Purpose

    Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven turbo green fluorescent protein
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. px330 EQR

    Plasmid

    #101733

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCoofy62

    Plasmid

    #122014

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Mammalian

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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