We narrowed to 59,461 results for: %s

1. pFX-GalT-mCherry

   Plasmid

   #174473

   Purpose

   Express mCherry with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Stra6L-6His

   Plasmid

   #174215

   Purpose

   Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

   Depositor

   Adrian Press

   Article

   Stafast et al ACS Appl. Polym. Mater. 2022

   Insert

   Stra6L (Stra6l Mouse)
   

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   non

   Promoter

   CMV

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSunS

   Plasmid

   #171672

   Purpose

   SunS expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Tags

   Strep II-MBP

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS-puro shNEDD4L #2

   Plasmid

   #27017

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   shRNA NEDD4L #2 (NEDD4L Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. WNK2 K207A

   Plasmid

   #24570

   Depositor

   Joseph Costello

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

   Insert

   WNK2` (WNK2 Human)
   

   Tags

   His6 and V5

   Expression

   Mammalian

   Mutation

   K207A

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pMSCV-puro-mMCL1-WT

   Plasmid

   #45817

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   mMCL-1
   

   Expression

   Mammalian

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-H2B-mCherry

   Plasmid

   #175841

   Purpose

   Express mCherry with nucleus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with nucleus-targeting sequence
   

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-mCherry-Rab5

    Plasmid

    #174455

    Purpose

    Express mCherry with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with early endosome-targeting sequence
    

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-GW182delta1

    Plasmid

    #11592

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    GW182 (TNRC6A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only aa1-498; SEE DEPOSITOR NOTES BELOW

    Available Since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pRCP

    Plasmid

    #195862

    Purpose

    Encodes chromogenic protein (CP). CP inducible with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Chromogenic Protein
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

    Plasmid

    #128609

    Purpose

    Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    uORF1-36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SchBE4max

    Plasmid

    #192135

    Purpose

    Expresses AncBE4max and nSchCas9

    Depositor

    Yongming Wang

    Article

    Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

    Insert

    AncBE4max, nSchCas9
    

    Expression

    Mammalian

    Mutation

    SchCas9 (D9A)

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Wnt8A

    Plasmid

    #35916

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt8A (Wnt8a Human)
    

    Expression

    Mammalian

    Mutation

    Silent Mutation A (600) --> G

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. StrepII-tag

    Plasmid

    #55181

    Purpose

    Streptactin-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    StrepII tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-L3-Csy4-Cas9n-EGFP-L2

    Plasmid

    #141046

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9n-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid

    #202568

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFU-MVIIA-PE

    Plasmid

    #24144

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

    Insert

    omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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