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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Expression

Bacterial

Promoter

pLacI

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_60

Plasmid

#130528

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-H1-gRNA

Plasmid

#61089

Purpose

CRISPR/Cas9 system gRNA cloning

Depositor

Shenglin Huang

Article

Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

Insert

H1 promoter; gRNA sequences

Use

CRISPR; /cas9 grna cloning vector

Expression

Mammalian

Promoter

H1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP7

Plasmid

#137910

Purpose

Constitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNA

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Insert

BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-C16orf80-flag

Plasmid

#70645

Purpose

Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C16orf80 (CFAP20 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRR-PR-5Z

Plasmid

#23057

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

PR (PGR Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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MBD2b pcDNA3.1

Plasmid

#78142

Purpose

Mammalian expression of MBD2b

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2b (MBD2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pBPGAL4.2::p65Uw

Plasmid

#26229

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::p65 (RELA Budding Yeast, Human)

Expression

Insect

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-ChR2-EGFP

Plasmid

#114216

Purpose

Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Channelrhodopsin 2

Use

AAV and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

CamKIIa 1.3 kb

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pKAd5f11p-EPG

Plasmid

#122697

Purpose

To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Promoter

EF1a

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pAGM38869

Plasmid

#153220

Purpose

Level 0 Arabidopsis U6-promoter vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis U6 snRNA promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-94N

Plasmid

#53281

Purpose

Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD28 SNIPR

Plasmid

#188358

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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