We narrowed to 18,937 results for: Tec

1. pAPM-miR30-LRPPRC-ts1

   Plasmid

   #174268

   Purpose

   LRPPRC knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   LRPPRC shRNA (LRPPRC Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPM-miR30-LRPPRC-ts2

   Plasmid

   #174269

   Purpose

   LRPPRC knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   LRPPRC shRNA (LRPPRC Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FNLS-PGK-Puro

   Plasmid

   #110844

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. STK11 gRNA (BRDN0001147319)

   Plasmid

   #75914

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK11

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK11 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. OCT4 GFP puro Donor 1

   Plasmid

   #22209

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDC43-FIT2

   Plasmid

   #96993

   Purpose

   Express GFP-tagged mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Tags

   GFP

   Expression

   Plant

   Mutation

   E2D (please see depositor comments below)

   Promoter

   35S

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLO-GFP-3UAG

   Plasmid

   #82501

   Purpose

   Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescence Protein with 3 UAG codons
   

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3, …

   Promoter

   AraBAD

   Available Since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX020

   Plasmid

   #167146

   Purpose

   MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal caffeoylpiruvate hydrolase, nnCPH
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid

    #113960

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-IBV-3CL

    Plasmid

    #160288

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

    Plasmid

    #160624

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2:VPR:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIM24 gRNA (BRDN0001146855)

    Plasmid

    #77886

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIM33 gRNA (BRDN0001162242)

    Plasmid

    #77099

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM33

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM33 (TRIM33 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. RPS6KA1 gRNA (BRDN0001145974)

    Plasmid

    #75497

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRIM33 gRNA (BRDN0001162291)

    Plasmid

    #77097

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM33

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM33 (TRIM33 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRIM33 gRNA (BRDN0001162239)

    Plasmid

    #77098

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM33

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM33 (TRIM33 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRIM24 gRNA (BRDN0001145637)

    Plasmid

    #77887

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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