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pM117: VEGFA array presgRNA

Plasmid

#166869

Purpose

U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting array presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-10

Plasmid

#160710

Purpose

For C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-8

Plasmid

#160708

Purpose

For N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-7

Plasmid

#160707

Purpose

For N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-27b

Plasmid

#112539

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-27b (MIR27B Human)

Use

Adenoviral

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Wnt2 STOP

Plasmid

#35869

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Gateway Cloning

Mutation

Silent Mutation G (816) --> A

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9-L2

Plasmid

#141038

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS(A) human p110 alpha E545K

Plasmid

#52211

Purpose

expresses human p110 alpha with E545K mutation in avian cells

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;10

Insert

p110 alpha E545K (PIK3CA Human)

Use

Retroviral; Avian

Mutation

Glutamic Acid 545 to Lysine

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt3A Non-STOP

Plasmid

#35890

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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Wnt1 Non-STOP

Plasmid

#35887

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGL-Lats2UTR

Plasmid

#20882

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Lats2 3'UTR (Lats2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-microRNA 146a-Dendra2

Plasmid

#97113

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 146a Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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miR-vector plix4.03

Plasmid

#97141

Purpose

For lentiviral tet-inducible expression of miRNA and Dendra2

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Type

Empty backbone

Use

Lentiviral

Tags

dendra2

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Wnt10A Non-STOP

Plasmid

#35902

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8B-V5

Plasmid

#35936

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1653_sgSOD1

Plasmid

#63711

Purpose

Express sgSOD1 in mammalian cells of human.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgSOD1, CAG promoter, mCherry, puro

Expression

Mammalian

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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PGRP-LCa

Plasmid

#21059

Depositor

Johann Deisenhofer

Article

Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

PGRP-LCa (PGRP-LF Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-Purg1-GST

Plasmid

#39352

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GST and kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only