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mNeonGreen-JIP4

Plasmid

#164621

Purpose

Expresses JIP4 in mammalian cells with a mNeonGreen tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

JIP4 (SPAG9 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE792

Plasmid

#167300

Purpose

Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162387

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Publication

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mApple

Expression

Mammalian

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Publication

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Peredox

Use

Gateway Cloning

Tags

mCherry

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ORF9b

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM2s2TsR

Plasmid

#137923

Purpose

E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

Depositor

Neel Joshi

Publication

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Publication

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

MSCV-LTR

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-PH-TAPP1

Plasmid

#161985

Purpose

Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

Depositor

Dario Alessi, David Bryant

Publication

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

GFP

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SARS-CoV-2_N_FL

Plasmid

#157867

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein

Depositor

Nicolas Fawzi

Publication

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Publication

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ExRai-AKAR2(T/A)

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-BLRR

Plasmid

#158958

Purpose

Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

Depositor

Charles P. Lai

Publication

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

BLRR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLX304_zeo_mmIrf1

Plasmid

#160098

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Publication

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDNA2.0-6H-TEV-KRAS G12D

Plasmid

#159439

Purpose

Expresses KRAS G12D in E.coli

Depositor

Kenneth Westover

Publication

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS G12D (KRAS Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-E29-ChR2GFP2x

Plasmid

#153437

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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