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AAV.CAG.GLPLight1

Plasmid

#187467

Purpose

Expresses GLPLight1 in mammalian cells

Depositor

Tommaso Patriarchi

Publication

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Promoter

CAG

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-TurboID-MCS

Plasmid

#186813

Purpose

CAG promoter-driven expression of TurboID-fused protein (N terminus)

Depositor

Masahito Ikawa

Publication

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

3xFLAG-TurboID

Expression

Mammalian

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Publication

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mito-ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Publication

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HiFi Cas9

Plasmid

#188490

Purpose

Expresses FLAG tagged HiFi Cas9

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R691A

Promoter

EF1a

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ10873

Plasmid

#183961

Purpose

U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

Depositor

Stanley Qi

Publication

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

HyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSprayerHE

Plasmid

#165150

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

Depositor

Paul Khavari

Publication

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BASU-GAL4

Expression

Mammalian

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PB-5'-ENGRAM

Plasmid

#179157

Purpose

piggyBac plasmid for 5' ENGRAM recorder

Depositor

Jay Shendure

Publication

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

minP

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Publication

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 nonmutant

Plasmid

#183813

Purpose

Express CD74-ROS1 fusion (C6;R34) in mammalian cells

Depositor

Aaron Hata

Publication

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Publication

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Publication

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Publication

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Publication

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hSyn-somBiPOLES-mCerulean

Plasmid

#154945

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Publication

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec23A

Plasmid

#166893

Purpose

mammalian expression of Sec23A tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Publication

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEC23A (SEC23A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOsU3

Plasmid

#170132

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDGE792

Plasmid

#167300

Purpose

Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-Sec31

Plasmid

#166895

Purpose

mammalian expression of Sec31 tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Publication

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec31

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSICO-eGFP-TNPO3

Plasmid

#167590

Purpose

Expresses human TNPO3 fused to eGFP

Depositor

Zandrea Ambrose

Publication

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

eGFP-TNPO3 (TNPO3 Human)

Use

Lentiviral

Tags

eGFP

Mutation

WT

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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