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pGWB535

Plasmid

#74873

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1)

Plasmid

#17452

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-multi-Guide

Plasmid

#85401

Purpose

Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcw107

Plasmid

#62511

Purpose

Gateway Expression Vector

Depositor

John Doench, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pYPQ202

Plasmid

#86198

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance marker

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Promoter

Arabidopsis ubiquitin 10 (AtUbi10)

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

Plasmid

#236863

Purpose

Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

NRAS (NRAS Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

Q61R

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGWB511

Plasmid

#74853

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Neo DEST (705-1)

Plasmid

#17392

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Pveg

Plasmid

#55173

Purpose

Very strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

veg promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

veg

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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