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pHH0103_VCL_Vinculin

Plasmid

#110045

Purpose

Protein expression and purification of VCL_Vinculin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCL_Vinculin (VCL Human)

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R365

Plasmid

#138235

Purpose

Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-CBX1wt-PA-mCherry

Plasmid

#138252

Purpose

Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64TS human TGFbeta-IIR (DM#169)

Plasmid

#15012

Depositor

Douglas Melton

Article

Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Insert

TGF beta type II receptor (TGFBR2 Human)

Use

Sp6 expression for use in xenopus

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS06

Plasmid

#52647

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS06

Expression

Mammalian

Mutation

M374Q mutated from GCaMP3

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pT2-cryR;sheP1Egfp

Plasmid

#90165

Purpose

contains She specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheP1Egfp (she Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMEsheP1gfp

Plasmid

#90130

Purpose

Gateway Mid-entry clone containing promoter sequence specific for She driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheP1Egfp (she Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_TOM1L2_VHS

Plasmid

#109901

Purpose

Protein expression and purification of TOM1L2_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TOM1L2_VHS (TOM1L2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS14

Plasmid

#52651

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS14

Expression

Mammalian

Mutation

deleted G47 from GCaMP3

Promoter

T7

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Cx26-7

Plasmid

#55473

Purpose

Localization: Gap Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Cx26

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
Ad-SNRK

Plasmid

#131539

Purpose

Expresses SNRK in mammalian cells

Depositor

Haiyan Xu

Article

Li et al Diabetes. 2013 Jul;62(7):2396-409. doi:

Insert

SNF related kinase (Snrk Mouse)

Use

Adenoviral

Tags

No

Mutation

No

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1 Cyclin D2 short UTR

Plasmid

#21644

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 short UTR (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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mTFP1-Profilin-10

Plasmid

#55506

Purpose

Localization: Cytoskeleton, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Profilin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP (F64L, S65T, V163A)-kanMX6

Plasmid

#53183

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

kanMX6

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
p3E-rab5b

Plasmid

#109718

Purpose

Multisite gateway vector for 3' tagging with rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab7b

Plasmid

#109724

Purpose

Multisite gateway vector for 3' tagging with rab7b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7b (rab7b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab11al

Plasmid

#109663

Purpose

Multisite gateway vector for 3' tagging with rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZF-ADD-L

Plasmid

#127477

Purpose

coding sequence for synthetic zinc finger ZF-ADD-L

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)

Use

Cell-free transcription–translation

Tags

PDZ cognate peptide ligand (VKESLV)

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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