Skip to main content
Addgene
Showing: 1351 - 1380 of 17340 results

  4. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232931-plasmid-map-sequence-id-466490

    Enlarge

  5. pJK637

    Plasmid
    #72437
    Purpose
    Acetobacter aceti 1023 mutS, partial gene
    Depositor
    T. J. Kappock
    Insert
    DNA mismatch repair protein MutS (partial gene)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deletes nt 1-767 and nt 2086-2655 of mutS gene
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72437-plasmid-map-sequence-id-132658

    Enlarge

  13. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

    Enlarge

  14. pDAC569

    Plasmid
    #195241
    Purpose
    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195241-plasmid-map-sequence-id-384464

    Enlarge

  20. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Promoter
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

    Enlarge

  21. pZJQIBEBT003

    Plasmid
    #140053
    Purpose
    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.
    Depositor
    Jia Zhang
    Article
    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952
    Insert
    eMutS-L157C-G233C-CBM-EGFP
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    L157C, G233C
    Promoter
    T7
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140053-plasmid-map-sequence-id-296195

    Enlarge

  25. pCSm5En2DER4(#1062)

    Plasmid
    #184093
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>D)-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    chkEn2: C175D; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184093-plasmid-map-sequence-id-365715

    Enlarge

  28. pT22i5uasH3En2SE4P3Che(#1212)

    Plasmid
    #184098
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    3xHA
    Expression
    Mutation
    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…
    Promoter
    5xUAS
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184098-plasmid-map-sequence-id-365869

    Enlarge

  29. pT22i5uasm5En25EE4P3Che(#1213)

    Plasmid
    #184099
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    5xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184099-plasmid-map-sequence-id-364013

    Enlarge

  30. pT22i5uasm5En2SE4P3Che(#1209)

    Plasmid
    #184097
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…
    Promoter
    5xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184097-plasmid-map-sequence-id-364012

    Enlarge

Showing: 1351 - 1380 of 17340 results