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Showing: 2251 - 2300 of 22937 results

  7. DONOR plasmid

    Plasmid
    #236552
    Purpose
    Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag
    Expression
    Mammalian
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236552-plasmid-map-sequence-id-469275

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  8. NoLS-Grx1-roGFP2

    Plasmid
    #236545
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    NoLS-Grx1-roGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236545-plasmid-map-sequence-id-469945

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  9. pMDW133

    Plasmid
    #227248
    Purpose
    Lambda DNA for torsional templates
    Depositor
    Michelle Wang
    Insert
    Lambda DNA
    Expression
    Bacterial
    Promoter
    Plac
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227248-plasmid-map-sequence-id-450969

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  10. NES-Grx1-roGFP2

    Plasmid
    #236551
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    NES-Grx1-roGFP2
    Tags
    nuclear export signal
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236551-plasmid-map-sequence-id-469946

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  11. Grx1-roGFP2-PSPC1

    Plasmid
    #236549
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in paraspeckles.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-PSPC1 (PSPC1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    236549-plasmid-map-sequence-id-475413

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  12. CRISPR plasmid

    Plasmid
    #236553
    Purpose
    Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Cas9
    Expression
    Mammalian
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236553-plasmid-map-sequence-id-469881

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  13. pXR004_puroR

    Plasmid
    #219820
    Purpose
    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219820-plasmid-map-sequence-id-441123

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  14. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  15. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  16. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  17. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

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  18. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  19. PKA1252

    Plasmid
    #239373
    Purpose
    GST-Sec4 (full length) cloned in BamH1 and Sal1 sites of pGEX-6P1
    Depositor
    Kathryn Ayscough
    Insert
    Sec4 (SEC4 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239373-plasmid-map-sequence-id-478753

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  20. OMM-SPLICSs-nFAST

    Plasmid
    #210430
    Purpose
    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    OMM-SPLICSs-nFAST
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210430-plasmid-map-sequence-id-415625

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  22. HaloTag-LacI

    Plasmid
    #232636
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232636-plasmid-map-sequence-id-462868

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  23. pLS406

    Plasmid
    #231078
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231078-plasmid-map-sequence-id-460262

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  24. pLS405

    Plasmid
    #231077
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231077-plasmid-map-sequence-id-459803

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  25. pLS404

    Plasmid
    #231076
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231076-plasmid-map-sequence-id-459800

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  26. pLS408

    Plasmid
    #231079
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231079-plasmid-map-sequence-id-459799

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  27. pLS403

    Plasmid
    #231075
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231075-plasmid-map-sequence-id-459792

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  28. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  29. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  30. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Expression
    Insect
    Promoter
    U6-3
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  32. pEZ-Frt-lox-DT

    Plasmid
    #11736
    Purpose
    Conditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.
    Depositor
    Klaus Rajewsky
    Insert
    pEZ-Frt-lox-DT
    Use
    Cre/Lox; /frt
    Available Since
    May 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11736-plasmid-map-sequence-id-7056

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  35. H2B-Grx1-roGFP2

    Plasmid
    #236550
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    H2B-Grx1-roGFP2 (H2BC21 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236550-plasmid-map-sequence-id-469939

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  39. pLS400

    Plasmid
    #231080
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX
    Depositor
    Sahand Jamal Rahi
    Article
    Scutteri et al bioRxiv 2024.11.05.622133
    Type
    Empty backbone
    Use
    Integrating
    Expression
    Bacterial and Yeast
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231080-plasmid-map-sequence-id-459791

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  43. G3BP1-Grx1-roGFP2

    Plasmid
    #236548
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    G3BP1-Grx1-roGFP2 (G3BP1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236548-plasmid-map-sequence-id-470046

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  45. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  47. HaloTag-LacI-KRAB

    Plasmid
    #232638
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-KRAB
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232638-plasmid-map-sequence-id-462856

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  48. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  49. Grx1-roGFP2-Dcp1b

    Plasmid
    #236547
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-Dcp1b (DCP1B Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236547-plasmid-map-sequence-id-469940

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  50. Grx1-roGFP2-Coilin

    Plasmid
    #236546
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in Cajal bodies.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-Coilin (COIL Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236546-plasmid-map-sequence-id-469938

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Showing: 2251 - 2300 of 22937 results