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We narrowed to 5,049 results for: golden gate

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Showing: 2251 - 2300 of 5049 results

  2. pMV_AA099

    Plasmid
    #245321
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD59
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD59 (CD59 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245321-plasmid-map-sequence-id-488022

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  6. pTE5451

    Plasmid
    #231066
    Purpose
    tetO-10xHis-phi29 DNA polymerase PovSak reporter: PT7-tetO-B0035-10xHis-p2 povsak-T7 terminator, AmpR
    Depositor
    Tobias Erb
    Article
    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/
    Insert
    PT7-tetO-B0035-10xHis-phi29 DNAP PovSak-T7term
    Expression
    Bacterial
    Promoter
    PT7-tetO
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    231066-plasmid-map-sequence-id-476591

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  13. pSF511

    Plasmid
    #222719
    Purpose
    CRISPR/Cas9 plasmid with gRNA1 for site gsdA, Cas9
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    gRNA1(gsdA)
    Use
    CRISPR; A. niger
    Expression
    Bacterial
    Promoter
    pmbfA
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    222719-plasmid-map-sequence-id-467742

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  14. pSF2

    Plasmid
    #222590
    Purpose
    gsdA disruption cassette via integration of pyrG from A. nidulans
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    pyrG
    Use
    A. niger
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222590-plasmid-map-sequence-id-443810

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  15. pAIP4_4IPC

    Plasmid
    #234229
    Purpose
    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4IPC (RPA1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234229-plasmid-map-sequence-id-464194

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  16. pAIP4_6F2Q

    Plasmid
    #234224
    Purpose
    Heterologous protein expression of galectin-3 (Gal-3) (CBP 35) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6F2Q (LGALS3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234224-plasmid-map-sequence-id-464189

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  17. pAIP4_2JHX

    Plasmid
    #234218
    Purpose
    Heterologous protein expression of rho GDP-dissociation inhibitor 1 (Rho GDI alpha) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2JHX (ARHGDIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234218-plasmid-map-sequence-id-464183

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  18. pAIP4_5HFD

    Plasmid
    #234189
    Purpose
    Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HFD (Dlg4 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234189-plasmid-map-sequence-id-464154

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  19. pAIP4_5MC7

    Plasmid
    #234155
    Purpose
    Heterologous protein expression of coatomer subunit zeta-1 (Zeta-1-coat protein) (Zeta-1 COP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5MC7 (COPZ1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234155-plasmid-map-sequence-id-464121

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  22. pVP293

    Plasmid
    #222062
    Purpose
    GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    Backbone Esp3i sites have been domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222062-plasmid-map-sequence-id-437925

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  23. pVP294

    Plasmid
    #222063
    Purpose
    GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    Backbone Esp3i sites have been domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222063-plasmid-map-sequence-id-447319

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  25. pVP076

    Plasmid
    #222028
    Purpose
    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222028-plasmid-map-sequence-id-447309

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  34. DVA_p15A_AG

    Plasmid
    #217603
    Purpose
    Destination vector with ampicillin resistance and p15A origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217603-plasmid-map-sequence-id-442585

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  35. DVA_p15A_EH

    Plasmid
    #217605
    Purpose
    Destination vector with ampicillin resistance and p15A origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217605-plasmid-map-sequence-id-442584

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  39. DVC_p15A_GH

    Plasmid
    #217591
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217591-plasmid-map-sequence-id-442566

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Showing: 2251 - 2300 of 5049 results