We narrowed to 4,045 results for: golden gate
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Plasmid#228231PurposeLevel 0 for 5xOp (GreenGate A module)DepositorInsert5xOp
UseSynthetic BiologyAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111111
Plasmid#222456PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-miniDFR (Solanum lycopersicum)Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111112
Plasmid#222457PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-miniDFRAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00015
Plasmid#222446PurposeLevel 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.DepositorInsertVP64-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91069
Plasmid#222462PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-miniDFR (Solanum lycopersicum)Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00014
Plasmid#222445PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.DepositorInsertVP16-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110034
Plasmid#222443PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.DepositorInsertLoxP-mCherry
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2142 MTK3-sfGFP
Plasmid#226661PurposeMTK3 part containing the coding sequence of sfGFPDepositorInsertsfGFP
UseSynthetic BiologyExpressionMammalianPromoternoneAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM034_P7_3"Lys3
Plasmid#216459Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM038_P8b_5'Lys3
Plasmid#216463Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2368 MTK PB puro backbone
Plasmid#226673PurposeEmpty MTK PB cargo backbone marked with puromycin resistance.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB67
Plasmid#226316PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with K to A CsoS2 MR1-6
ExpressionBacterialMutationK261A, K320A, K380A, K436A, K495A, K546APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB74
Plasmid#226314PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-5
ExpressionBacterialMutationY300A, Y359A, Y420A, Y475A, Y534APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB73
Plasmid#226313PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1-3
ExpressionBacterialMutationY300A, Y359A, Y420APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB72
Plasmid#226312PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with Y to A CsoS2 MR1
ExpressionBacterialMutationY300APromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB45
Plasmid#226304PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S CsoS2 MR1-2
ExpressionBacterialMutationC292S, C310S, C351S, C369SPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB44
Plasmid#226303PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with C to S MR1
ExpressionBacterialMutationC292S, C310SPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB43
Plasmid#226302PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertWT CsoS2, frameshift site intact
ExpressionBacterialPromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1426
Plasmid#218622PurposeExpress tNCS to the peroxisome for toxicity assayDepositorInserttNCS
TagsYFP-ePTS1ExpressionYeastMutation106-588Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1462
Plasmid#218623PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV proteaseDepositorInsertYFP
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1463
Plasmid#218624PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV proteaseDepositorInsertYFP
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1734
Plasmid#218627PurposeExpress amorphadiene synthase (ADS)DepositorInsertADS
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1535
Plasmid#218613PurposeExpress Pex5DepositorInsertPex5
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1536
Plasmid#218617PurposeExpress Pex5 and Pex11DepositorInsertPex5
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1376
Plasmid#218618PurposeExpress Pex5, Pex3, Pex19, and Pex8DepositorInsertPex5
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1338
Plasmid#218592PurposeExpress a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22DepositorInsertPex22
ExpressionYeastMutationPex22(1-36)Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB207
Plasmid#218596PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
TagsUbiY degradation tag and ePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1469
Plasmid#218588PurposeExpress peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonateDepositorInsertMvaS
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5417_p3WJdB_promoter dropout vector
Plasmid#221191PurposePromoter probe vector with dropout sequence for promoter sequences with 3WJdB reporterDepositorTypeEmpty backboneUseSynthetic BiologyPromoterPromoter Placeholder/Dropout VectorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
int>pro::GFP
Plasmid#218576PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationInsert genomic sequence 41-372nt to -46nt upstrea…Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
int>UTR::GFP
Plasmid#218577PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationInsert genomic sequence 41-372nt to -622nt upstre…Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔ4):GFP
Plasmid#218569PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationDeletion genomic sequence from 254nt-343ntAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔ3):GFP
Plasmid#218568PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationDeletion genomic sequence from 182nt-271ntAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔ2):GFP
Plasmid#218567PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationDeletion genomic sequence from 132nt-221ntAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔ1):GFP
Plasmid#218566PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationDeletion genomic sequence from 58nt-147ntAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM040_P8b_5'Ura4
Plasmid#216461Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'ura4
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM036_P7_3"Ura4
Plasmid#216465Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''ura4
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM024_P2_pMPA1
Plasmid#216449PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter mpa1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM022_P2_pRPS1002
Plasmid#216447PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rps1002
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM020_P2_pTRS1
Plasmid#216445PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter trs1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM014_P2_pGPM1
Plasmid#216439PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter gpm1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM042_P15678_MultiGeneBackbone
Plasmid#216467PurposeEmpty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM041_P15678_SingleGeneBackbone
Plasmid#216466PurposeEmpty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM032_P4_tSynth30
Plasmid#216457PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth30
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM031_P4_tSynth29
Plasmid#216456PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth29
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM030_P4_tSynth27
Plasmid#216455PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth27
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM029_P4_tSynth25
Plasmid#216454PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth25
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM028_P4_tSynth3
Plasmid#216453PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only