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pLenti-CMV-NFE2L2-Puro

Plasmid

#181919

Purpose

Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Tags

Citrine and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG RABV-G

Plasmid

#36398

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

rabies virus glycoprotein

Promoter

CAGGs

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pXL004-ishRNA-scramble

Plasmid

#36311

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of scramble

Use

Lentiviral and RNAi

Tags

EF1a-TetR-IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4_WT_OCT4

Plasmid

#40629

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pEYFP-Synectin

Plasmid

#35791

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
3580 pcDNA3 Bad S112A S136A

Plasmid

#8799

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S112A, S136A

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-YF-UniRapR-mCerulean-myc

Plasmid

#45381

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-YF-UniRapR-mCerulean-myc

Tags

Myc, Myr, and mCerulean

Expression

Mammalian

Mutation

changed mSrc Tyrosine 529 to Phenylalanine

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher EQ

Plasmid

#35045

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher EQ

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1-(151-241)

Plasmid

#24393

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 151-241 are deleted. Delta CC mutant.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 K44A

Plasmid

#128153

Purpose

Expression of Dynamin 2 K44A

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

Insert

Dynamin 2 (Dnm2 Rat)

Expression

Mammalian

Mutation

K44A

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

S727A

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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