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Showing: 901 - 920 of 65322 results

  2. pGEM_ΔndhD2.km

    Plasmid
    #185532
    Purpose
    a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    nptII gene flanked by ndhD2 upstream and downstream regions
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185532-plasmid-map-sequence-id-368416

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  3. pGEM_Δflv3.sm

    Plasmid
    #185533
    Purpose
    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    aadA gene flanked by flv3 upstream and downstream regions
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185533-plasmid-map-sequence-id-371409

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  4. pACBE-FSR (pXK-1648)

    Plasmid
    #208821
    Purpose
    Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.
    Depositor
    Kun Xu
    Article
    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi
    Type
    Empty backbone
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208821-plasmid-map-sequence-id-417246

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  5. BPTI-21

    Plasmid
    #195058
    Purpose
    simplyfied amino acid sequence by alanine scanning
    Depositor
    Yutaka Kuroda, Peter Kim
    Article
    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d
    Insert
    bovine pancreatic trypsin inhibitor
    Use
    Tags
    His tag and trpΔLE leader sequences
    Expression
    Bacterial
    Mutation
    21 amino acids are Alanine out of 58 amino acids
    Promoter
    T7 promotor
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195058-plasmid-map-sequence-id-388061

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  6. pSilencer2.1-U6-T1-U6-bc339

    Plasmid
    #190849
    Purpose
    For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Inserts
    T1 (an RNA aptamer of O-GlcNAc Transferase)
    bc339 (an RNA aptamer of β-catenin)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190849-plasmid-map-sequence-id-379246

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  8. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  11. pAC-EHER

    Plasmid
    #53262
    Purpose
    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53262-plasmid-map-sequence-id-82988

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  20. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Article
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    no
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59981-plasmid-map-sequence-id-96247

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Showing: 901 - 920 of 65322 results