We narrowed to 61,534 results for: promoter
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Plasmid#60240PurposeIPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.DepositorInserttnpA from pBam1
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7 promoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST17-UbE2D3
Plasmid#15784DepositorPublicationInsertUbiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
TagsHisExpressionBacterialMutationsee Depositor Comments belowAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorTypeEmpty backboneTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP6f
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40
Plasmid#100835PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas serviceAAV1, AAV2, AAV5, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9_D10A
UseCRISPRExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-Jaws-KGC-GFP-ER2
Plasmid#65014PurposeAAV-mediated expression of Jaws under the human synapsin promoterDepositorHas serviceAAV5, AAV8, and AAV RetrogradeInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterhuman synapsinAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Plasmid#100842PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
c-myc promoter (4x TBE2 - wt)
Plasmid#16599DepositorPublicationAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE
Plasmid#104492PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV9, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Igfbp1-promoter/pGL3
Plasmid#12146DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIGFBP1 promoter (Igfbp1 Rat)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains the canonical insulin response sequence …Available sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130995PurposeAAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoterDepositorHas serviceAAV8InsertChRmine
UseAAVTagsmScarlet-Kv2.1PromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEFCas9
Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralExpressionMammalianPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E-cadherin-GFP
Plasmid#28009DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertE-cadherin (CDH1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 17, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1-TFEB
Plasmid#38119PurposeMammalian expression of Transcription factor EB fused to EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFEB (TFEB Human)
TagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by