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Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)
Plasmid#111255PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptideDepositorInsertTorT signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-NG1Tt
Plasmid#44523DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPGal1 (wt)Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLMB708
Plasmid#83782PurposeTartrate biosensor plasmidDepositorInsertRegion upstream RL0996 containing all of RL0997
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB710
Plasmid#83783PurposeFormate biosensor plasmidDepositorInsertRegion upstream RL4393 (fdsG) containing all of RL4394
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB712
Plasmid#83784PurposeHesperetin biosensor plasmidDepositorInsertRegion upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB705
Plasmid#83787PurposeMannitol biosensor plasmidDepositorInsertRegion upstream RL4218 (mtlB) containing all of RL4219
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB717
Plasmid#83788PurposeErythritol biosensor plasmidDepositorInsertRegion upstream pRL90085 containing all of pRL90087
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB737
Plasmid#83790PurposeMalonate biosensor plasmidDepositorInsertRegion upstream RL0990 (matA) containing all of RL0989 (matR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB738
Plasmid#83791PurposeGABA biosensor plasmidDepositorInsertRegion upstream RL0102 (gabT) containing all of RL0103
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MMP2AG-T2APuroR-5h
Plasmid#128256PurposeMammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.DepositorInsertrtTA-Advanced::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT234_(pCA-G-intron-tTA2)
Plasmid#36874DepositorInsertsGFP
tTA2
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-5
Plasmid#233294PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionMutationPromoterp14G-BCD2Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterP-LlacO-1Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-TT3
Plasmid#173418PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNG-NG dimer RVD at Position3
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-TT4
Plasmid#173419PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNG-NG dimer RVD at Position4
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-TT5
Plasmid#173420PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNG-NG dimer RVD at Position5
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-TT6
Plasmid#173421PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNG-NG dimer RVD at Position6
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-TT7
Plasmid#173422PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNG-NG dimer RVD at Position7
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only