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pFBOH-MHL.RBBP4

Plasmid

#125173

Purpose

Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-TEV site

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-HaloTag

Plasmid

#181953

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdc2-AF (718)

Plasmid

#39872

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Mutation

AF = T14A, Y15F

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)

Plasmid

#235005

Purpose

Bacterial expression of N-terminally 6His tagged E3 ubiquitin-protein ligase (Topors) LCD

Depositor

Tanja Mittag

Insert

E3 ubiquitin-protein ligase (Topors) (TOPORS Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-CRBN-pGK-HYG

Plasmid

#107380

Purpose

Mammalian expression of FLAG-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD

Plasmid

#235577

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-GFP

Plasmid

#61215

Purpose

Retrovirus expressing GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Retroviral

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ233 (dLbCpf1-SRDX)

Plasmid

#86211

Purpose

Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR

Tags

5' and 3' NLS and SRDX repressor

Expression

Plant

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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ptRNA100

Plasmid

#154133

Purpose

Expresses a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

tRNAs operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pflareG-psd95-exon18

Plasmid

#90266

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

Psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-Bag6

Plasmid

#61836

Purpose

express N-terminally FLAG tagged human Bag6

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

Bag6 (BAG6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

Plasmid

#100054

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6.DC-FLAG

Plasmid

#30483

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Catalytically inactive mutant (H216/611A)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pmCherry-RPA32

Plasmid

#211382

Purpose

mammalian expression of human RPA32 N-terminally fused to mCherry

Depositor

Helmut Pospiech

Insert

RPA32 (RPA2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Venus-rab5a

Plasmid

#234742

Purpose

Mammalian expression of early endosome marker Venus-rab5a

Depositor

Nevin Lambert

Article

Lan et al PLoS One. 2011 Feb 22;6(2):e17361.

Insert

Venus-rab5a (RAB5A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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