165,652 results

1. XPO6-EGFP

   Plasmid

   #210371

   Purpose

   Expresses XPO6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPOTv7-hygro-3HaloTag

   Plasmid

   #179828

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   3x HaloTag
   

   Use

   Unspecified

   Promoter

   read through

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJFRC-MUH

   Plasmid

   #26213

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nsp13-mCherry

   Plasmid

   #165136

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_NESPylRS(WT)_hU6tRNAPyl

   Plasmid

   #223509

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Expression

   Mammalian

   Promoter

   CMV, SP6 and U6

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-CD63-L7Ae

   Plasmid

   #180503

   Purpose

   Human CD63-L7Ae fusion protein

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Human CD63-L7Ae fusion protein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

    Plasmid

    #210017

    Purpose

    episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2A61V is a point mutant of Sox2 that enhances …

    Promoter

    CAG

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE60 R130A wgMDH

    Plasmid

    #204266

    Purpose

    Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GFP-CD63

    Plasmid

    #194989

    Purpose

    overexpress CD63

    Depositor

    Angelique Bordey

    Insert

    GFP-CD63
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRE-Tn7-165

    Plasmid

    #118577

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRANS_250d

    Plasmid

    #91124

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    PvUbi2

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS2 (hANGPTL4-V5)

    Plasmid

    #102446

    Purpose

    Expresses V5-tagged human ANGPTL4 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    ANGPTL4 (ANGPTL4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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