We narrowed to 11,803 results for: nsf

1. AAV_Nanog HMEJ donor

   Plasmid

   #97321

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Nanog HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-SynTetOff-myrGFP

   Plasmid

   #190233

   Purpose

   AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   myristoylation signal sequence

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP-shTACC3

   Plasmid

   #59356

   Purpose

   Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GACh2.0

   Plasmid

   #121921

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor GACh2.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-HA-deLACCO1-NGR

   Plasmid

   #208097

   Purpose

   Control biosensor for extracellular L-lactate

   Depositor

   Robert Campbell, Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   deLACCO1
   

   Use

   AAV

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

   Plasmid

   #120293

   Purpose

   AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   N-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   SV40-NLS and split-intein

   Expression

   Mammalian

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

   Plasmid

   #194692

   Purpose

   hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-Syn-ArchT-GFP

   Plasmid

   #63141

   Purpose

   AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   human synapsin promoter

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKII-iGECI-NES

    Plasmid

    #160424

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EFS-DjCas13d-HA-mCherry-pA

    Plasmid

    #233030

    Purpose

    To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH-Ubi-miniSdd7-nCas9-PBE

    Plasmid

    #204856

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1, OsU3, 35S

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLgw-MBD3-V5-Dam

    Plasmid

    #55605

    Purpose

    Expression of Dam fused MBD3 in mammalian cells

    Depositor

    Paul Wade

    Article

    Shimbo et al PLoS Genet. 2013 Dec;9(12):e1004028. doi

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Lentiviral

    Tags

    DamID (DNA adenine methyltransferase identificati…

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

    Plasmid

    #176281

    Purpose

    Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBacMUT-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    E191K

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

    Plasmid

    #196414

    Purpose

    Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smFLAG
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-FLEX-ArgiNLS-AausFP1

    Plasmid

    #220602

    Purpose

    Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUT8-Dox

    Plasmid

    #124631

    Purpose

    Doxycycline-inducible FUT8 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    

    Expression

    Mammalian

    Promoter

    TRE-tight and hEF1a

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-gfaABC1D-R-iLACCO1.1

    Plasmid

    #208103

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    AAV

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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