20,361 results
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Plasmid#82478PurposemicroRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.DepositorInsertH2B-mCherry and H2B-Citrine. HygroR
Available sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY095
Plasmid#84744PurposeExpresses huLbCpf1-T2A-GFP and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshuLbCpf1
GFP
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pP{ELAV-GeneSwitch}
Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertExpressionInsectPromoterelavAvailable sinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIPZ (M)-HT-Cbx2
Plasmid#82510PurposeExpresses Halotag-Cbx2 fusion proteins in mammalian cellsDepositorInsertChromobox Homolog 2 (CBX2 Human)
UseLentiviralTagsHaloTagExpressionMammalianPromoteraggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…Available sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sg-A
Plasmid#83807PurposeExpress sgRNA targeting SA siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSgRNA
UseCRISPRAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-loxP-mTagBFP2-stop-pA-loxP
Plasmid#75158PurposeFloxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.DepositorPublicationInsertloxP-mTagBFP2-stop-pA-loxP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS4B_Flag
Plasmid#79640PurposeThird generation Self-inactivating lentivector expressing NS4B from Zika virusDepositorInsertNS4B
UseLentiviralTagsFlagExpressionMammalianPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-smURFP-T2A-mCardinal
Plasmid#80348PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.DepositorInsertssmURFP
mCardinal
UseLentiviralPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPICZalphaB-SapL3
Plasmid#78171PurposeExpression of Saporin L3 in pichiaDepositorInsertSaporin L3
Tagsalpha factorExpressionYeastAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAT FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKanCMV-mRuby3-18aa-actin
Plasmid#74255PurposeMammalian expression of N-terminal fusion of mRuby3 to actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertactin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUGW-FerH-ffLuc2-eGFP
Plasmid#71393PurposeLentiviral vector of luciferase-eGFP fusion gene driven by FerH promoterDepositorInsertFerH-ffLuc2-eGFP
UseLentiviralExpressionMammalianPromoterFerHAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-IRF9
Plasmid#71452PurposeLentiviral expression of IRF9DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pERB264
Plasmid#67764PurposeTags peroxisomes with GFP and HalotagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEX3-GFP-Halo
TagsGFP and HalotagExpressionMammalianAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only