162,408 results

1. 2XMyc-LRRK2-WT

   Plasmid

   #25361

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-U6-sgRNA-CMV-GFP

   Plasmid

   #85451

   Purpose

   Express sgRNA in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pCMV-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCMV-EGFP

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. CH-GECO2.1

   Plasmid

   #52099

   Purpose

   mCherry-based Calcium indicators

   Depositor

   Robert Campbell

   Article

   Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

   Insert

   CH-GECO2.1
   

   Use

   Tags

   6 His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promotor

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   David Sabatini, Eric Lander

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin and U6

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Parkin

   Plasmid

   #23956

   Purpose

   Mammalian expression of human Park2 fused to mCherry

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Park2 (PRKN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ICUE3

   Plasmid

   #61622

   Purpose

   FRET-based biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP and cpVenus[L194]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    human OCT4

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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