159,557 results

1. pGL3-TGFb1

   Plasmid

   #101762

   Purpose

   TGFb1 promoter reporter

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   transforming growth factor beta 1 promoter (TGFB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_BRAF_p.V600E

   Plasmid

   #81700

   Purpose

   Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   V600E

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-CD63-L7Ae

   Plasmid

   #180503

   Purpose

   Human CD63-L7Ae fusion protein

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   Human CD63-L7Ae fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Drp1K38A

   Plasmid

   #45161

   Depositor

   Alexander van der Bliek, Richard Youle

   Article

   Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   HA (AYPYDVPDY)

   Expression

   Mammalian

   Mutation

   K38A

   Promoter

   CMV

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO-puro-tetON-shRFP

   Plasmid

   #110940

   Purpose

   Tet-inducible shRNA targeting RFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shRFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Expression

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. PKD1 gRNA (BRDN0001148399)

   Plasmid

   #76842

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKD1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKD1 (PKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTXB1-nbOcIgG-Tn5

   Plasmid

   #184285

   Purpose

   Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   Ivan Raimondi, New York Genome Center

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbOcIgG-Tn5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF1844

   Plasmid

   #144313

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RB1 (RB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid

    #141347

    Purpose

    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

    Depositor

    Gerald Pao

    Article

    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

    Insert

    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    resynthesized with human codon optimization nucle…

    Promoter

    CAG

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-2T PTP-1B

    Plasmid

    #8602

    Depositor

    Ben Neel

    Insert

    PTP-1B (PTPN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-SKP1-T131A

    Plasmid

    #213482

    Purpose

    Expresses human SKP1 T131A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-T131A (SKP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFlagCMV2-EFP

    Plasmid

    #12449

    Depositor

    Dong-Er Zhang

    Article

    Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

    Insert

    EFP (TRIM25 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p236

    Plasmid

    #186182

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 236
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA5/FRT-ChemoG-ATP

    Plasmid

    #193818

    Purpose

    CMV overexpression of ChemoG-ATP in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-ninein C-ter

    Plasmid

    #73523

    Purpose

    Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein C-ter (residues 1874-2113) (Nin Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge