165,652 results

1. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3 HA eIF4GI (1-1599)

   Plasmid

   #45640

   Depositor

   Nahum Sonenberg

   Article

   Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

   Insert

   eIF4GI transcript variant 2 (EIF4G1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_hSyn1_nLightG

   Plasmid

   #187179

   Purpose

   Expresses green norepinephrine indicator nLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Has Service

   AAV9

   Insert

   nLightG
   

   Use

   AAV

   Tags

   Flag tag

   Promoter

   human Synapsin-1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti.CAG.H2B-Dendra2.W

   Plasmid

   #51005

   Purpose

   Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

   Depositor

   Rusty Lansford

   Insert

   H2B-Dendra2
   

   Use

   Lentiviral

   Tags

   histone2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LARS_pNIC-Bio3

   Plasmid

   #153052

   Purpose

   Expression construct for producing Avi tagged Leucyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   LARS (LARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Not full length protein

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCI SMN2

   Plasmid

   #72287

   Purpose

   SMN2 exon 7 splicing cassette

   Depositor

   Elliot Androphy

   Article

   Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

   Insert

   SMN2 (SMN2 Human)
   

   Expression

   Mammalian

   Mutation

   contains exons 6-7-8 including introns, multiple …

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmCherry-RIAM

    Plasmid

    #80030

    Purpose

    Mammalian expression of mCherry-RIAM (FL)

    Depositor

    Chinten James Lim

    Insert

    RIAM (APBB1IP Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST44

    Plasmid

    #64007

    Purpose

    polycistronic cloning vector for pST44 plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Xl-VSP1

    Plasmid

    #51880

    Purpose

    expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223_CBR1_WT_V5

    Plasmid

    #82945

    Purpose

    Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CBR1 (CBR1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Promoter

    cx55.5

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge