Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,247 results for: multi

Clear keywords
Showing: 9261 - 9280 of 18247 results

  1. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid
    #209060
    Purpose
    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.
    Depositor
    Matthew Oser
    Publication
    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    Use
    Gateway vector to be used for lr reaction
    Promoter
    U6
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209060-plasmid-map-sequence-id-412264

    Enlarge

  3. pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)

    Plasmid
    #189794
    Purpose
    Express GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells
    Depositor
    Robert Singer
    Publication
    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta-globin (HBB Human, Mouse)
    Expression
    Mammalian
    Promoter
    Ponasterone A inducible
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    189794-plasmid-map-sequence-id-381422

    Enlarge

  6. pEF1α-M2rtTA-T2A-Puro

    Plasmid
    #169175
    Purpose
    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174
    Depositor
    Igor Slukvin
    Publication
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    M2rtTA-T2A-Puro
    Use
    Piggybac transposon
    Expression
    Mammalian
    Mutation
    See Depositor Comments Below
    Promoter
    EF1a
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169175-plasmid-map-sequence-id-362474

    Enlarge

  8. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

    Enlarge

  10. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Publication
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

    Enlarge

  11. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid
    #132554
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    Expression
    Mammalian
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132554-plasmid-map-sequence-id-259533

    Enlarge

  13. pAAV-UBC-S1-jRCaMP1a

    Plasmid
    #160728
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Publication
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-jRCaMP1a
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160728-plasmid-map-sequence-id-315534

    Enlarge

  17. Mouse 5' Npm1 AID GFP PuroR

    Plasmid
    #127899
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene
    Depositor
    Mark Groudine
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Npm1 (Npm1 Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127899-plasmid-map-sequence-id-250993

    Enlarge

  18. pFGL1258

    Plasmid
    #116898
    Purpose
    TetOFF:GFP:CenpA (centromere marker)
    Depositor
    Naweed Naqvi
    Publication
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CenpA promoter region
    CenpA
    Use
    Fungal expression (in magnaporthe oryzae)
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    116898-plasmid-map-sequence-id-232123

    Enlarge

  19. pMTG06-Amp-FRT

    Plasmid
    #128275
    Purpose
    Deg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFP
    Depositor
    Gabor Balazsi
    Publication
    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TetR-LOV-RRRG
    EGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128275-plasmid-map-sequence-id-251906

    Enlarge

  20. pLaNGo11-7

    Plasmid
    #126055
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Publication
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126055-plasmid-map-sequence-id-247152

    Enlarge

Showing: 9261 - 9280 of 18247 results